Seminar "DNA barcode technologies for high-throughput measurements of molecular and cellular dynamics" Prof. Nozomu Yachie

Speaker: Nozomu Yachie

Associate Professor, Research Center for Advanced Science and Technology

Title: DNA barcode technologies for high-throughput measurements of molecular and cellular dynamics

Abstract: Beyond its impact on personal genome sequencing, massively parallel DNA sequencing has enabled various high-throughput assays with the idea of DNA barcode. In this talk, I will first summarize high-throughput experiments enabled by DNA barcodes and introduce Barcode Fusion Genetics (BFG) technology that we developed for en masse phenotyping of heterogeneous cell pools where each cell has a different combination of two or more genetically engineered loci. We combined this technology with Yeast Two-Hybrid and screened various protein interactomes from up to ~2.5 million protein pairs in 2-3 weeks. I will also talk about our recent efforts towards high-resolution lineage tracing of dynamic cell population using DNA barcode and genome editing technologies and their potential applications to study cell evolution and development.