FY2014 Annual Report

Cell Signal Unit

Professor Tadashi Yamamoto

Abstract

 

1. Staff

  • Dr.Toru Suzuki, Group leader
  • Dr.Naosuke Hoshina, Postdoctoral Scholar
  • Dr.Yo-taro Shirai, Postdoctoral Scholar
  • Dr.Taku Kureha, Postdoctoral Scholar
  • Dr.Akinori Takahashi, Postdoctoral Scholar
  • Dr.Kristopher Paraiso Montrose, Postdoctoral Scholar
  • Ms.Miyuki Hoshina, Technical Staff
  • Ms.Chisato Kikuguchi, Technical Staff
  • Ms.Xue Li, Technical Staff
  • Ms.Miho Tokumasu, Technical Staff
  • Ms.Aja Sanzone, Technical Staff
  • Ms.Sandrine Anne Laure  Burriel-Ha, Graduate Student
  • Ms.Kaori Yamashiro, Research Administrator

2. Collaborations

  • Theme: Physiological studies of the CCR4-NOT complex
    • Type of collaboration: Joint research
    • Researchers:
      • Kuba K and Imai Y. Department of Physiology, Graduate School of Medicine, Akita University
  • Theme: Structural analysis of the CCR4-NOT complex
    • Type of collaboration:Joint research
    • Researchers:
      • Bartlam M and Rao Z. College of Life Sciences, Nankai University, China 
  • Theme: Molecular mechanism of mRNA degradation mediated by the CCR4-NOT deadenylase
    • Type of collaboration: Joint research
    • Researchers:
      • Morita M, Fabian M, and Sonenberg N. Department of Biochemistry and Goodman Cancer Research Center, McGill University, Canada 
  • Theme: Bioinformatics of gene expression affected by impairment of mRNA degradation machinery
    • Type of collaboration: Joint research
    • Researchers:
      • Nagashima T and Okada M. Laboratory for Cellular System Modeling, RIKEN Research Center for Allergy and Immunology
  • Theme: Roles of the Fyn tyrosine kinase and its substrates in the central nervous system
    • Type of collaboration: Joint research
    • Researchers:
      • Nakazawa T and Kano M. Department of Neurophysiology, Graduate School of Medicine, University of Tokyo
  • Theme: Roles of the CNOT3 subuni of the CCR4-NOT complex in B cell development
    • Type of collaboration: Joint research
    • Researchers:
      • Inoue T and Kurosaki T. Immunology Frontier Research Center, Osaka University

3. Activities and Findings

 

 

4. Publications

4.1 Journals

1. Shirai, Y. T., Suzuki, T., Morita, M., Takahashi, A. & Yamamoto, T.  Multifunctional roles of the mammalian CCR4-NOT complex in physiological phenomena. Frontiers in genetics 5, 286, doi:10.3389/fgene.2014.00286

2. Knox, R., Brennan-Minnella, A. M., Lu, F. X., Yang, D. N., Nakazawa, T., Yamamoto, T., Swanson, R. A., Ferriero, D. M. & Jiang, X. N.  NR2B Phosphorylation at Tyrosine 1472 Contributes to Brain Injury in a Rodent Model of Neonatal Hypoxia-Ischemia. Stroke 45, 3040-3047, doi:Doi 10.1161/Stroke aha.114.006170

3. Ito-Kureha, T., Koshikawa, N., Yamamoto, M., Semba, K., Yamaguchi, N., Yamamoto, T., Seiki, M. & Inoue, J.  Tropomodulin 1 Expression Driven by NF-kappaB Enhances Breast Cancer Growth. Cancer research 75, 62-72, doi:10.1158/0008-5472.CAN-13-3455

4. Inoue, T., Hoshina, N., Nakazawa, T., Kiyama, Y., Kobayashi, S., Abe, T., Yamamoto, T., Manabe, T. & Yamamoto, T.  LMTK3 deficiency causes pronounced locomotor hyperactivity and impairs endocytic trafficking. J Neurosci 34, 5927-5937, doi:10.1523/JNEUROSCI.1621-13.2014

5. David, O., Barrera, I., Chinnakkaruppan, A., Kaphzan, H., Nakazawa, T., Yamamoto, T. & Rosenblum, K.  Dopamine-induced tyrosine phosphorylation of NR2B (Tyr1472) is essential for ERK1/2 activation and processing of novel taste information. Frontiers in molecular neuroscience 7, 66, doi:10.3389/fnmol.2014.00066

4.2 Books and other one-time publications

Nothing to report

4.3 Oral and Poster Presentations

1. Zukeran, A.  The efficient generation of iPS cells from MEFs by CCR4-NOT complex, in 2nd CCR-4NOT Research Conference, OIST (2014)

2. Yamamoto, T.  Tob/CCR4-NOT complex and regulation of energy metabolism., in Medical Chemistry for Next Horizen, Inamori Hall (2015).

3. Yamamoto, T.  Creation of disease models by disruption of mRNA metabolism homeostasis, in 3rd CCR4-NOT Research Conference, Sakan (2015).

4. Yamamoto, T.  Roles of CNOT3, a component of the CCR4-NOT Complex, in post-transcriptional regulation, in 2nd CCR4-NOT Research Conference, Cincinnati Children's Hospital Medical Center (2014).

5. Yamamoto, T.  CCR4-NOT-mediated mRNA decay: Mechanism and functional implication, in China-Japan Joint Laboratory Workshop 2014, Institute of Biophysics Chinese Academy of Sciences (2014).

6. Yamamoto, T.  From oncogenes to cell signaling, in The 73rd Annual Meeting of the Japanese Cancer Association, Pacifico Yokohama (2014).

7. Yamamoto, T.  CCR4-NOT-mediated mRNA Decay: Mechanism and Functional Implication, in 16th RNA meeting, Wink Aichi (2014).

8. Yamamoto, T.  Impairment of the control of homeostasis through disruption of CCR4-NOT deadenylase function, The Institute of Medical Science The University of Tokyo (2014).

9. Yamamoto, T.  Roles of CNOT3, a component of the CCR4-NOT Complex, in post-transcriptional regulation, Cincinnati Children's Hospital Medical Center (2014).

10. Yamamoto, T.  The LMTK family kinases in endocytic membrane traffic, in Nikon Imaging Center at Harvard Medical School Imaging Symposium, Harvard Medical School (2014).

11. Tsubasa Ohhashi, T. Y., Yuji Yamanashi, Miho Ohsugi.  ɤ-tubulin2 exhibit lower microtubule dynamics than ɤ-tubulin1 and is insufficient to form bipolar spindle, in The 37th Annual Meeting of the Molecular Biology Society of Japan, Pacifico Yokohama (2014).

12. Tsubasa Ohhashi, T. Y., Yuji Yamanashi, Miho Ohsugi.  ɤ-tubulin2 exhibit lower microtubule dynamics than ɤ-tubulin1 and is insufficient to form bipolar spindle, in The 37th Annual Meeting of the Molecular Biology Society of Japana, Pacifico Yokohama (2014).

13. Tsubasa Ohashi, T. Y., Yuji Yamanashi, Miho Ohsugi.  γ-tubulin2 is functionally different from γ-tubulin1 in cancer cells., in EMBO Conference: Centrosomes and spindle pole bodies, Calouste Gulbenkian Foundation, Lisbon, Portugal (2014)

5. Intellectual Property Rights and Other Specific Achievements

Nithing to report.

6. Meetings and Event

6.1 Seminar

Title: Genome-wide analysis of hormone signaling in prostate and breast cancer
Date: Wednesday, April 2nd, 2014
Venue: C016, Lab1
Speaker: Prof. Satoshi Inoue
Department of Anti-Aging Medicine, Graduate School of Medicine, The University of Tokyo
Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University

6.2 Seminar

Title: NF-kB, a key player in breast cancer development
Date: Wednesday, April 9th, 2014
Venue: C016, Lab1
Speaker: Prof. Junichiro Inoue
Division of Cellular and Molecular Biology, Department of Cancer Biology
Institute of Medical Science, University of Tokyo

6.3 Seminar

Title: Chasing a single amino acid substitution in HIV-1 circulating among Japanese population.
Date: Friday, April 18th, 2014
Venue: C016, Lab1
Speaker: Prof. Aikichi Iwamoto
The University of Tokyo The Institute of Medical Science
Advanced Clinical Research Center Division of Infectious Diseases, Research Hospital Division of Infectious Diseases and Applied Immunology

6.4 Seminar

Title: Neuroscience using iPS cell technologies and Transgenic non-human primates
Date: Friday, July 4th, 2014
Venue: C016, Lab1
Speaker: Prof. Hideyuki Okano
Professor, Dean, Keio University Graduate School of Medicine
Professor & Chair, Department of Physiology, Keio University, School of Medicine

6.5 Seminar

Title: Structural biology of clathrin-mediated endocytosis
Date: Monday, July 7th, 2014
Venue: C016, Lab1
Speaker: Dr. Atsushi Shimada
Division of Structural Biology, Medical Institute of Bioregulation, Kyushu University

6.6 Seminar

Title: Reverse-genetic approach to dissect the function of abundant nuclear long noncoding RNAs
Date: Friday, August 1st, 2014
Venue: C015, Lab1
Speaker: Dr. Shinichi Nakagawa
Associate Chief Scientist, RIKEN, Nakagawa RNA biology Laboratory, Saitama, Japan

6.7 Seminar

Title: NKX2-1 and its downstream target, SCGB3A2: good cop or bad cop in lung cancer?
Date: Monday, September 1st, 2014
Venue: C015, Lab1
Speaker: Dr. Shioko Kimura
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA

6.8 Seminar

Title: Dissection of Branching Morphogenesis and Patterning Mechanisms Underlying
Neurovascular Wiring during Organogenesis
Date: Monday, September 8th, 2014
Venue: C015, Lab1
Speaker: Prof. Yosuke Mukoyama
Principal Investigator, Laboratory of Stem Cell and Neuro-Vascular Biology
Genetics and Developmental Biology Center
National Heart, Lung, and Blood Institute
National Institutes of Health

6.9 Seminar

Title: Wiring the Functional Brain
Date: Monday, October 6th, 2014
Venue: C015, Lab1
Speaker: Prof. Hisashi Umemori
F.M. Kirby Neurobiology Center, Boston Children's Hospital, Department of Neurology, Harvard Medical School
Boston, MA, USA

6.10 Seminar

Title: An Impact of GTP-Metabolism on Cancers and Metabolic Diseases
Date: Monday, December 1st, 2014                                        
Venue: C016, Lab1
Speaker: Dr. Atsuo T. Sasaki
University Cincinnati Cancer Institute

6.11 Seminar

Title: Modeling Epstein-Barr-Virus (EBV) pathologies and immune surveillance against virus-driven lymphoma
Date: Tuesday, December 16th, 2014
Venue: C016, Lab1
Speaker: Dr. Tomoharu Yasuda
Max Delbruck Center for Molecular Medicine, Berlin

6.12 Seminar

Title: Novel roles of ribosome ubiquitination in quality control systems
Date: Monday, December 22nd, 2014
Venue: C016, Lab1
Speaker: Prof. Toshifumi Inada
Professor, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai

6.13 Seminar

Title: Therapeutic strategies targeting cancer stem cells
Date: Thursday, February 12, 2015
Venue: C209, Center Building
Speaker: Hideyuki Saya
Division of Gene Regulation, Institute for Advanced Medical Research (IAMR)
Keio University School of Medicine, Tokyo Japan

6.14 Seminar

Title: Roles of a mitochondrial respiratory supercomplex assembly factor in muscle, fat and cancer metabolism
Date: Wednesday, February 25th, 2015
Venue: C016, Lab1
Speaker: Prof. Satoshi Inoue
Department of Anti-Aging Medicine, The University of Tokyo, Japan
Dvision of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Japan

6.15 Seminar

Title: Regulation of the TRAF6-NF-kB-NFATc1 signal pathway in osteoclastogenesis
Date: Wednesday, March 25th, 2015
Venue: C016, Lab1
Speaker: Prof. Jun-ichiro Inoue
Division of Cellular and Molecular Biology, Department of Cancer Biology, Institute of Medical Science, University of Tokyo

6.16 The 2nd CCR4-NOT Meeting

  • Date: June 24-27, 2014
  • Venue: C600, Lab 2, OIST
  • Organizer: Tadashi Yamamoto (OIST)
  • The number of Participants: 25

 6.17 The 3rd CCR4-NOT Meeting

  • Date: March 13-15, 2015
  • Venue: Sakan, Sendai, Miyagi
  • Organizer: Toshifumi Inada (Tohoku University)
  • The number of Participants: 20

 6.18 RNA Meeting

  • Date: July 31- Sugust 2, 2014
  • Venue: OIST Seaside House
  • Organizer: Tadashi Yamamoto (OIST)
  • The number of Participants: 9