Seminar "DNA barcode technologies for high-throughput measurements of molecular and cellular dynamics" Prof. Nozomu Yachie

Date

Wednesday, February 8, 2017 - 14:00 to 15:00

Location

C016, Lab 1

Description

Speaker: Nozomu Yachie

Associate Professor, Research Center for Advanced Science and Technology

 

Title: DNA barcode technologies for high-throughput measurements of molecular and cellular dynamics

 

Abstract: Beyond its impact on personal genome sequencing, massively parallel DNA sequencing has enabled various high-throughput assays with the idea of DNA barcode. In this talk, I will first summarize high-throughput experiments enabled by DNA barcodes and introduce Barcode Fusion Genetics (BFG) technology that we developed for en masse phenotyping of heterogeneous cell pools where each cell has a different combination of two or more genetically engineered loci. We combined this technology with Yeast Two-Hybrid and screened various protein interactomes from up to ~2.5 million protein pairs in 2-3 weeks. I will also talk about our recent efforts towards high-resolution lineage tracing of dynamic cell population using DNA barcode and genome editing technologies and their potential applications to study cell evolution and development.

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