Peer Reviewed Articles


22.      Lee, R.X.*, Stephens, G.J., & Kuhn B.  Behavioural development during post-traumatic stress incubation in adult mice. (under review)

21.      Mori, K* & Kuhn, B.*  Imaging Ca2+ concentration and pH in nanopores/channels of protein crystals. J.Phys.Chem.B 122, 42, 9646-9653 (2018)

20.      Roome, C.J. * & Kuhn, B.*   Simultaneous dendritic voltage and calcium imaging and somatic recording from Purkinje neurons in awake mice.   Nature communications  DOI: 10.1038/s41467-018-05900-3 (2018)

OIST Press Release

19.      Lissek, T., ...., & Hasan, M.T.*   Building Bridges through Science.   NeuroView in Neuron 96(4), p.730-735 (2017)

OIST Press Release

18.      Funamizu, A., Kuhn, B., & Doya, K.*   Neural substrate of dynamic Bayesian inference in the cerebral cortex.   Nature Neuroscience 19, p.1682–1689 (2016).

OIST Press Release


17.      Roome C.J.* & Kuhn B.*   Chronic cranial window with access port for repeated cellular manipulations, drug application, and electrophysiology.   Frontiers in Cellular Neuroscience 8:379, doi: 10.3389/fncel.2014.00379 (2014).

16.       Augustinaite, S., Kuhn, B., Helm, P. J. & Heggelund, P.*    NMDA Spike/Plateau Potentials in Dendrites of Thalamocortical Neurons.   The Journal of Neuroscience 34, 10892-10905, doi:10.1523/JNEUROSCI.1205-13.2014 (2014).

Cover of The Journal of Neuroscience 34    

Cover of The Journal of Neuroscience Special Issue for Japan Neuroscience Society Meeting Attendees

Covered in AlphaGalileo and BioOptics World and SPIE Newsroom and on the OIST web page

15.         Seemann, K. M.* & Kuhn, B.*    Multi-photon excited luminescence of magnetic FePt core-shell nanoparticles.   Biomedical Optics Express 5, 2446-2457, doi:10.1364 (2014).

14.         Anwar, H., Roome, C. J., Nedelescu, H., Chen, W., Kuhn, B. & De Schutter, E.*    Dendritic diameters affect the spatial variability of intracellular calcium dynamics in computer models.   Frontiers in Cellular Neuroscience 8, article 168, doi:10.3389/fncel.2014.00168 (2014).

13.         Huang, S. & Uusisaari, M. Y.*    Physiological temperature during brain slicing enhances the quality of acute slice preparations.   Frontiers in Cellular Neuroscience 7, 1-8, doi:doi: 10.3389/fncel.2013.00048 (2013).

Covered on the OIST web page

12.         Seemann, K. M.*, Kiefersauer, R., Jacob, U. & Kuhn, B.*    Optical pH detection within a protein crystal.   J Phys Chem B 116, 9873-9881, doi:10.1021/jp2103512 (2012).

Covered on the OIST web page

11.         Kuhn, B., Ozden, I., Lampi, Y., Hasan, M. T. & Wang, S. S.-H.*    An amplified promoter system for targeted expression of calcium indicator proteins in the cerebellar cortex.   Front Neural Circuits 6, article 49, doi:10.3389/fncir.2012.00049 (2012).

Covered on the OIST web page

10.         Kuhn, B., Hoogland, T. M. & Wang, S.S.-H.*    Cerebellar craniotomy for in vivo calcium imaging of astrocytes.   Cold Spring Harb Protoc 10, 1224-1227, doi:10.1101/pdb.prot065805. (2011).

9.         Kuhn, B., Hoogland, T. M. & Wang, S.S.-H.*    Injection of recombinant adenovirus for delivery of genetically encoded calcium indicators into astrocytes of the cerebellar cortex.   Cold Spring Harb Protoc 10, 1217-1223, doi:10.1101/pdb.prot065797. (2011).

8.         Hoogland, T. M., Kuhn, B. & Wang, S.S.-H.*    Preferential loading of bergmann glia with synthetic acetoxymethyl calcium dyes.   Cold Spring Harb Protoc 10, 1228-1231, doi:10.1101/pdb.prot065813. (2011).

7.       Granstedt, A. E., Kuhn, B., Wang, S. S.-H. & Enquist, L. W.*    Calcium imaging of neuronal circuits in vivo using a circuit-tracing pseudorabies virus.   Cold Spring Harb Protoc 2010, pdb prot5410, doi:10.1101/pdb.prot5410 (2010).

6.       Hoogland, T. M.*, Kuhn, B.*, Göbel, W., Huang, W., Nakai, J., Helmchen, F., Flint, J. & Wang, S.S.-H.*    Radially expanding transglial calcium waves in the intact cerebellum.   Proc Natl Acad Sci U S A 106, 3496-3501, doi:10.1073/pnas.0809269106 (2009).

Selected for "In this issue", PNAS

5.       Granstedt, A. E., Szpara, M. L., Kuhn, B., Wang, S. S.-H & Enquist, L. W.*    Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus.   PLoS One 4, e6923, doi:10.1371/journal.pone.0006923 (2009).

4.       Kuhn, B.*, Denk, W. & Bruno, R. M.*    In vivo two-photon voltage-sensitive dye imaging reveals top-down control of cortical layers 1 and 2 during wakefulness.   Proc Natl Acad Sci U S A 105, 7588-7593, doi:10.1073/pnas.0802462105 (2008).

Covered in "Neurobiology Select", Cell

3.       Fromherz, P., Hübener, G., Kuhn, B. & Hinner, M. J.    ANNINE-6plus, a voltage-sensitive dye with good solubility*, strong membrane binding and high sensitivity.   European biophysics journal : EBJ 37, 509-514, doi:10.1007/s00249-007-0210-y (2008).

2.       Kuhn, B.*, Fromherz, P. & Denk, W.    High sensitivity of Stark-shift voltage-sensing dyes by one- or two-photon excitation near the red spectral edge.   Biophysical journal 87, 631-639, doi:10.1529/biophysj.104.040477 (2004).

1.       Kuhn, B. & Fromherz, P.*    Anellated hemicyanine dyes in a neuron membrane: Molecular Stark effect and optical voltage recording.   Journal of Physical Chemistry B 107, 7903-7913, doi:Doi 10.1021/Jp0345811 (2003).


Book Chapters


4.     B. Kuhn & C.J. Roome (in press) Voltage imaging with ANNINE dyes and two-photon microscopy. In: Multi-photon microscopy. E. Hartveit (Editor). Book series Springer-Nature Neuromethods.

3.    H. Anwar, C.J. Roome, H. Nedelescu, W. Chen, B. Kuhn, and E. De Schutter (2016) Dendritic diameters affect the spatial variability of intracellular calcium dynamics in computer models. In: Determinants of Synaptic Information Transfer: from Ca2+ Binding Proteins to Ca2+ Signaling Domains. P. Isope, C.D. Wilms, H. Schmidt (Editors). Frontiers Media SA, p. 112-125.

2.    Kuhn, B., Hoogland, T. M. & Wang, S.S.-H. (2011) In vivo calcium imaging of cerebellar astrocytes with synthetic and genetic indicators.  In Imaging in Neuroscience (2nd Edition).   F. Helmchen, A. Konnerth, & R. Yuste (Editors). Cold Spring Habor Laboratory Press, p. 707-720.

1.    Theer, P., Kuhn, B., Keusters, D. & Denk, W. (2005) Two-photon microscopy and imaging.   In Encyclopaedia of Molecular Cell Biology and Molecular Medicine. R.A. Meyers (Editor). Vol. 15,  p. 61-88.




3.       Hoogland, T. M. & Kuhn, B.    Recent Developments in the Understanding of Astrocyte Function in the Cerebellum In Vivo.   Cerebellum 9, 264-271, doi:DOI 10.1007/s12311-009-0139-z (2010).

2.       Hoogland, T. M., Civillico, E. F. & Kuhn, B.    Molecular layer Interneurons relay cerebellar cortical activity to bergmann glial cells.   Journal of Neuroscience 27, 11167-11169, doi:Doi 10.1523/Jneurosci.3597-07.2007 (2007).

1.       P. Theer, B. Kuhn, D. Keusters, W. Denk (2006) Two‐photon Microscopy and Imaging.  Reviews in Cell Biology and Molecular Medicine, Wiley‐VCH Verlag GmbH & Co. KGaA





2.    Kuhn, B. & Roome, C. J.    Chronic Cranial Window Allowing Drug Application and Electrophysiology  (2014).

1.    Kuhn, B., Hübener, G., Fromherz, P. & Denk, W.    Optimized determination of voltage-changes using a voltage-sensitive dye  (2004).