The reason why miRNAs bind to CDS

Dear All,

Cell Signal Unit (Yamamoto Unit) would like to inform you of a seminar by Dr. Hiroaki Sako from the University of Tokyo.

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Date: Friday, December 13, 2019

Time: 15:00-16:00

Venue: D015, Level C, Lab 1

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Speaker:

Dr. Hiroaki Sako from the University of Tokyo

Title:
The reason why miRNAs bind to CDS

Abstract:
Why most of miRNAs bind to CDS is yet to be understood. Recent CLIP data suggest that CDS and 3´UTR are equally targeted by miRNAs. mRNA degradation or translational suppression are triggered mainly by the miRNAs associated with 3´UTR. Although some of the CDS-binding miRNAs negatively regulate gene expression, many of them have weak or trivial effect compared to ones in 3´UTR.

Translational elongation rates are not constant. Ribosomes move faster or slower. The factors controlling elongation rates are such as codon optimality, amino acid availability,  mRNA secondary structure, or phosphorylation state of elongation factors. Slow elongation rates prevent nascent protein from misfolding. This could be achieved by more HSP70 recruited to unfolded nascent peptides during ribosomes moving slowly.

Our analysis of ribosome profiling and publicly available CLIP data suggest and we speculate that CDS-binding miRNAs “pause” translating ribosomes and help the nascent peptides to be correctly folded. I also present some preliminary wet data supporting the idea.

Host:
Prof. Tadashi Yamamoto

We hope to see many of you at the seminar.

Best regards,

Yuki Nakagawa

Research Unit Administrator

Cell Signal Unit