Electron Microscopy
Imaging Section (IMG-request*oist.jp) (replace* by @)
Imaging Section (IMG-request*oist.jp) (replace* by @)
Image Corrected Environmental Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Titan ETEM
Features:
Acc. Voltage: 80 - 300 keV
Holder: Gatan Heating Holder Single Tilt/Double Tilt, Gatan Cooling Holder, Fischione Tomography Holder, Protochips Aduro (Heating and Biasing Holder), Protochips Poseidon (Liquid Cell Holder with heating, biasing, and flow).
Camera: Ceta IS, Quantum ER Energy Filter.
Software: TEM/STEM Tomography Software, SerialEM, Holoworks.
Other equipment: STEM (BF/DF/HAADF detector), Oxford X-Max EDX detector, Biprism.
A device capable of observing atoms with high resolution, TEM mode (with Cs correction function) and STEM mode, an EDX detector and an EELS detector. The ETEM allows observation of samples in gaseous environments with a range of in-situ holders.
Atomic Resolution Analytical Transmission Electron Microscope
Manufacturer: JEOL
Model: JEM-ARM200F
Features:
Acc. Voltage: 60 - 200 keV
A device capable of observing atoms with high resolution, TEM mode and STEM mode (with Cs correction function), an EDX detector and an EELS detector. (Scanning resolution: 0.078 nm)
Transmission Electron Microscope (TEM)
Manufacturer: JEOL
Model: JEOL1400 Flash
Features:
Acc. Voltage: 100 keV
An electron microscope that uses a beam of electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.
Software: Limitless Panorama, for automated montaging of images.
Cryo-Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Titan Krios
Features:
Acc. Voltage: 300 keV
Holder:
Cryo-stage/Autoloader plan 3
Camera:
FEI Falcon 3EC, FEI Ceta camera, Gatan energy filter with Gatan K2
Software:
EPU automated data acquisition software, TEM/STEM Tomography software, SerialEM, Leginon
Other equipments:
Phase plate, STEM (BF/DF/HAADF detector)
Cryo-Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Talos Arctica
Features:
Acc. Voltage: 200 keV
Holder:
Cryo-stage/Autoloader plan 3
Camera:
FEI Falcon 3EC, FEI Ceta camera
Software:
EPU automated data acquisition software, TEM/STEM Tomography software, SerialEM, Leginon
Cryo-Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Talos L120C
Features:
Acc. Voltage:120 keV
Holder:
Single-tilt holder, high-field-of-view tomography holder, cryo-holder (Fischione, Model 2550)
Camera:
FEI Ceta camera
Software:
EPU automated data acquisition software, TEM Tomography software, SerialEM
Serial Block-Face Scanning Electron Microscope (SBEM)
Manufacturer: Thermo Fisher Scientific
Model: Teneo-VS
Features:
The Teneo has a build-in microtome that allows the removal of a thin layer of resin with subsequent imaging of the remaining block-face to acquire high-resolution data of large volumes. Generally the volumes are larger and the resolution is lower that with a FIB-SEM.
This SEM can also be used for array tomography.
Scanning Electron Microscope (SEM)
Manufacturer: JEOL
Model: JSM-7900F
Features:
A device is capable of SEM imaging in high vacuum and low vaccume mode with mutiple detectors from low accelerating voltage (100 V)
The device is also equippped with 2 set of EDX detectors for elemental analysis and EBSD for crystal phase analysis.
Focused Ion Beam / Scanning Electron Microscope (FIB-SEM)
Manufacturer: Thermo Fisher Scientific
Model: Helios NanoLab 650
Features:
The Helios is equipped with a dual beam system: a focused gallium ion beam (FIB) for sample processing and an electron beam (SEM) for simultaneous imaging.
Our system is mainly used for FIB-SEM tomography of resin embedded biological samples.
A cryo-stage from Quorum Company, UK, allows to process and image cryo-samples.