FY2020 Cryo-Electron Microscopy Course at OIST
Date
Location
Description
Organizer : Bruno M. Humbel, Okinawa Institute of Science and Technology
Venue : Okinawa Institute of Science and Technology
Aim :
This course on cryo-electron microscopy is aimed at beginners. Experienced researchers will provide an overview of the current state of the art of this Nobel Prize winning technologies and share their experiences in demonstrations and hands-on practice.
Opening Talk : Matthias Wolf (Molecular Cryo-Electron Microscopy Unit, OIST)
Keynote Lecture (on Zoom) : Jacques Dubochet (Winner of the Nobel Prize in Chemistry 2017, an Honorary Professor of Biophysics at the University of Lausanne in Switzerland)
Invited Instructors:
| Radostin Danev (the University of Tokyo) | Yoshiyuki Fukuda (the University of Tokyo) | Yoshimasa Takizawa (the University of Tokyo) |
| Takashi Yokoyama (Tohoku University) | Yukihiko Sugita (Kyoto University) | Naruhiko Adachi (KEK) |
| Kazuyoshi Murata (NIPS) | Toshio Moriya (KEK) | Kaoru Mitsuoka (Osaka University) |
| Takayuki Kato (Osaka University) | Junichi Kishikawa (Osaka University) | Kazuhiro Aoyama (Osaka University) |
|
Instructors from OIST : Malgorzata Hall & Ryo Kanno (Imaging Section) |
|
|
Schedule (tentative) |
|
| Day 1 Monday, February 15th |
| Lecture & Presenation @ B250 | Hands-on (Pre-registered members only) |
| Lecture: Sample preparation (Takeshi Yokoyama) | G1: Basic training on Talos Arctica / Cryo sample preparation |
| Short presentation by participants | G2: Cryo sample preparation / Negative staining sample preparation |
| G3: Negative staining sample preparation / Basic training on Talos Arctica |
| Day 2 Tuesday, February 16th | |
| Lecture & Presentation B250 | Hands-on (Pre-registered members only) |
| Presentation: Challenges for the chromatin structure determination by cryo-EM in 2021 (Yoshimasa Takizawa) | G1: Nagative staining sample screening on Talos L120C /Negative staining sample preparation |
| Lecture: Electron optics and image formation (Radostin Danev) | G2: Cryo sample screening on Talos Arctica / Basic training of Talos Arctice |
| Presentaion: Cryo-EM at Kyoto University (Yukihiko Sugita) | G3: Automated data collection on Titan Krios / Cryo sample preparation |
| Day 3 Wednesday, February 17th | |
| Lecture & Presenation @ B250 | Hands-on (Pre-registered members only) |
| Lecture: Hardware and development of cryo-TEM (Takayuki Kato) | G1: Cryo sample screening on Talos Arctica / Automated data collection on Titan Krios |
| Keynote Lecture : A Nobel prize, how and why ? (Jacques Dubochet) | G2: Automated data collection on Titan Krios / Negative staining sample screening on Talos L120C |
| G3: Nagative staining sample screening on Talos L120C / Cryo sample screening on Talos Arctica | |
| Day 4 Thursday, February 18th | |
| Lecture & Presenation @ Lab3 C700 | Hands-on (Pre-registered members only) |
| Lecture: Image Processing and theory (Kaoru Mitsuoka) | All Groups: Relion Image Processing #1 /#2 |
| Presentation: Cryo-EM at Okazaki (Kazuyoshi Murata) | |
| Presentation: Workflow of cryo electron tomography (Yoshiyuki Fukuda) | |
| Day 5 Friday, February 19th | |
| Presentation @ C210 | Hands-on (Pre-registered members only) |
| Presentation: Beyond Relion3.1 Tutorial (Toshio Moriya) | All Groups: Relion Image Processing #3 / #4 |
| Presentation: Cryo-EM support at OIST (Ryo Kanno) | |
|
Remarks:
|
We have stopped accepting applications for cryo-electron microscopy course.
*Registration is required for Hands-on session ONLY*
*We will contact you by the beginning of January if you proceed to participate in our course*
Contact: OIST Imaging Section (IMG-request@oist.jp)
Subscribe to the OIST Calendar: Right-click to download, then open in your calendar application.