FY2020 Cryo-Electron Microscopy Course at OIST

Date

Monday, February 15, 2021 - 09:00 to Friday, February 19, 2021 - 18:00

Location

Center Buidling C210/Lab 1 B503/Lab3 C700 and IMG Lab

Description

Organizer :  Bruno M. Humbel, Okinawa Institute of Science and Technology

Venue : Okinawa Institute of Science and Technology

Aim :

This course on cryo-electron microscopy is aimed at beginners. Experienced researchers will provide an overview of the current state of the art of this Nobel Prize winning technologies and share their experiences in demonstrations and hands-on practice.

Opening Talk : Matthias Wolf  (Molecular Cryo-Electron Microscopy Unit, OIST)

Keynote Lecture (on Zoom) : Jacques Dubochet (Winner of the Nobel Prize in Chemistry 2017, an Honorary Professor of Biophysics at the University of Lausanne in Switzerland)

Invited Instructors:

Radostin Danev (the University of Tokyo) Yoshiyuki Fukuda (the University of Tokyo) Yoshimasa Takizawa (the University of Tokyo)
Takashi Yokoyama (Tohoku University) Yukihiko Sugita (Kyoto University) Naruhiko Adachi (KEK)
Kazuyoshi Murata (NIPS) Toshio Moriya (KEK) Kaoru Mitsuoka (Osaka University)
Takayuki Kato (Osaka University) Junichi Kishikawa (Osaka University) Kazuhiro Aoyama (Osaka University)

Instructors from OIST :

Malgorzata Hall & Ryo Kanno (Imaging Section)

 

Schedule (tentative)

 
   
Day 1  Monday, February 15th  
Lecture & Presenation @ Center Building C210 Hands-on (Pre-registered members only)
Lecture: Sample preparation G1: Basic training on Talos Arctica  / Cryo sample preparation
Short presentation by participants G2: Cryo sample preparation /  Negative staining sample preparation
  G3: Negative staining sample preparation / Basic training on Talos Arctica
   
Day 2  Tuesday, February 16th  
Lecture & Presentation @ Center Building C210 Hands-on (Pre-registered members only)
Presentation : TBA G1: Nagative staining sample screening on Talos L120C /Negative staining sample preparation
Lecture : Electron optics and image formulation G2: Cryo sample screening on Talos Arctica / Basic training of Talos Arctice
  G3:  Automated data collection on Titan Krios / Cryo sample preparation
   
   
   
Day 3  Wednesday, February 17th  
Lecture & Presenation @ Center Building C210 Hands-on (Pre-registered members only)
Lecture: Image Processing and theory G1: Cryo sample screening on Talos Arctica / Automated data collection on Titan Krios
Keynote Lecture : TBA G2: Automated data collection on Titan Krios / Negative staining sample screening on Talos L120C
  G3:  Nagative staining sample screening on Talos L120C / Cryo sample screening on Talos Arctica
   

 

Day 4  Thursday, February 18th  
Lecture & Presenation @ Lab3 C700 Hands-on (Pre-registered members only)
Presentation: TBA All Groups: Relion Image Processing #1 / #2
Lecture: Hardware and development of cryo-TEM  
   
   
Day 5  Friday, February 7th  
Presentation @ Lab1 B503 Hands-on (Pre-registered members only)
Presentation: TBA All Groups: Relion Image Processing #3 / #4
   
   

Remarks:

 

Registration: https://groups.oist.jp/img/registration-form-cryo-electron-course-oist

*Registration is required for Hands-on session ONLY*

 

*We will contact you by the beginning of January if you proceed to participate in our course*

Contact: OIST Imaging Section (IMG-request@oist.jp)

All-OIST Category: 

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