FY2019 Cryo-Electron Microscopy Course at OIST
Date
Location
Description
Organizer : Bruno M. Humbel, Okinawa Institute of Science and Technology
Venue : Okinawa Institute of Science and Technology
Aim :
This course on cryo-electron microscopy is aimed at beginners. Experienced researchers will provide an overview of the current state of the art of this Nobel Prize winning technologies and share their experiences in demonstrations and hands-on practice.
Keynote Lecture : Holger Stark (Director at the Max-Planck-Institute for Biophysical Chemistry, Department of Structural Dynamics)
Invited Instructors:
| Radostin Danev (the University of Tokyo) | Yoshiyuki Fukuda (the University of Tokyo) | Yoshimasa Takizawa (the University of Tokyo) |
| Takashi Yokoyama (Tohoku University) | Yukihiko Sugita (Kyoto University) | Takayuki Kato (Osaka University) |
| Kazuyoshi Murata (NIPS) | Hideki Shigematsu (RIKEN SPring-8 Center) | Kaoru Mistsuoka (Osaka University) |
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Assistant Instructor : Naruhiko Adachi (KEK) Instructors from OIST : Ryusuke Kuwahara (Quantum Wave Microscopy Unit) Malgorzata Hall & Ryo Kanno (Imaging Section) |
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Schedule : you can download the final version here |
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| Day 1 Monday, February 3rd | |
| Morning Session | Afternoon Session |
| Open Lecture & Presenation @ Lab3 C700 | Hands-on (Pre-registered members only) |
| Lecture: Basics of cryo-TEM (Kaoru Mitsuoka) | G1: Negative staining sample preparation & Basic training on Talos L120C /Cryo sample preparation |
| Presentation: TBA (Kazuyoshi Murata) | G2: Basic training on Talos Arctica / Negative staining sample preparation & Basic training on Talos L120C |
| G3: Cryo sample preparation / Basic training on Talos Arctica |
| Day 2 Tuesday, February 4th | |
| Open Lecture @ Lab1 B503 | Keynote Lecture @ Lab3 C700 |
| Lecture: Hardware and Optics (Takayuki Kato) | Keynote: TBA (Holger Stark) |
| Hands-on (Pre-registered members only) | Hands-on (Pre-registered members only) |
| G1: Basic training on Talos Arctica | G1: Negative staining sample screening on Talos L120C |
| G2: Cryo sample preparation | G2: Cryo sample screening on Talos Arctica |
| G3: Negative staining sample preparation & Basic training on Talos L120C | G3: Automated data collection on Titan Krios |
| Day 3 Wednesday, February 5th | |
| Open Lecture & Presenation @ Lab1 C503 | Hands-on (Pre-registered members only) |
| Lecture: Image reconstruction (Hideki Shigematsu) | G1: Cryo sample screening on Talos Arctica / Automated data collection on Titan Krios |
| Presentation: TBA (Yukihiko Sugita) | G2: Automated data collection on Titan Krios / Negative staining sample screening on Talos L120C |
| Presentation: TBA (Takeshi Yokoyama) | G3: Negative staining sample screening on Talos L120C / Cryo sample screening on Talos Arctica |
| Day 4 Thursday, February 6th | |
| Open Lecture & Presenation @ Lab1 C503 | Hands-on (Pre-registered members only) |
| Presentation: TBA (Yoshimasa Takizawa) | All Groups: Relion Image Processing #1 / #2 |
| Lecture: Advanced techniques of cryo-EM (Radostin Danev) | |
| Presentation: TBA (Yoshiyuki Fukuda) | |
| Day 5 Friday, February 7th | |
| Open Presentation @ Lab1 B503 | Hands-on (Pre-registered members only) |
| Presentation: TBA (Kanno & Hall from Imaging Section, OIST) | All Groups: Relion Image Processing #3 / #4 |
Registration: https://groups.oist.jp/img/registration-form-
We have stopped accepting applications for cryo-electron microscopy course.
*Registration is required for Hands-on session ONLY*
*Registration is required for Hands-on session ONLY*
*We will contact you by the beginning of January if you proceed to participate in our course*
Contact: Mizuki Tokashiki, OIST Imaging Section (mizuki.tokashiki@oist.jp)
Subscribe to the OIST Calendar: Right-click to download, then open in your calendar application.