FY2019 Cryo-Electron Microscopy Course at OIST

Date

Monday, February 3, 2020 - 09:00 to Friday, February 7, 2020 - 18:00

Location

Lab 1 B503/Lab3 C700 and IMG Lab

Description

Organizer :  Bruno M. Humbel, Okinawa Institute of Science and Technology

Venue : Okinawa Institute of Science and Technology

Aim :

This course on cryo-electron microscopy is aimed at beginners. Experienced researchers will provide an overview of the current state of the art of this Nobel Prize winning technologies and share their experiences in demonstrations and hands-on practice.

Keynote Lecture : Holger Stark (Director at the Max-Planck-Institute for Biophysical Chemistry, Department of Structural Dynamics)

Invited Instructors:

Radostin Danev (the University of Tokyo) Yoshiyuki Fukuda (the University of Tokyo) Yoshimasa Takizawa (the University of Tokyo)
Takashi Yokoyama (Tohoku University) Yukihiko Sugita (Kyoto University) Takayuki Kato (Osaka University)
Kazuyoshi Murata (NIPS) Hideki Shigematsu (RIKEN SPring-8 Center) Kaoru Mistsuoka (Osaka University)

Assistant Instructor :

Naruhiko Adachi (KEK)

Instructors from OIST :

Ryusuke Kuwahara (Quantum Wave Microscopy Unit)

Malgorzata Hall & Ryo Kanno (Imaging Section)

 

Schedule : you can download the final version here

 
Day 1  Monday, February 3rd  
Morning Session Afternoon Session
Open Lecture & Presenation @ Lab3 C700 Hands-on (Pre-registered members only)
Lecture: Basics of cryo-TEM (Kaoru Mitsuoka) G1: Negative staining sample preparation & Basic training on Talos L120C /Cryo sample preparation
Presentation: TBA (Kazuyoshi Murata) G2: Basic training on Talos Arctica / Negative staining sample preparation & Basic training on Talos L120C
  G3: Cryo sample preparation / Basic training on Talos Arctica
   
Day 2  Tuesday, February 4th  
Open Lecture @ Lab1 B503 Keynote Lecture @ Lab3 C700
Lecture: Hardware and Optics  (Takayuki Kato) Keynote: TBA (Holger Stark)
Hands-on (Pre-registered members only) Hands-on (Pre-registered members only)
G1: Basic training on Talos Arctica G1: Negative staining sample screening on Talos L120C
G2: Cryo sample preparation G2: Cryo sample screening on Talos Arctica
G3: Negative staining sample preparation & Basic training on Talos L120C G3: Automated data collection on Titan Krios
   
Day 3  Wednesday, February 5th  
Open Lecture & Presenation @ Lab1 C503 Hands-on (Pre-registered members only)
Lecture: Image reconstruction (Hideki Shigematsu) G1: Cryo sample screening on Talos Arctica / Automated data collection on Titan Krios
Presentation: TBA (Yukihiko Sugita) G2: Automated data collection on Titan Krios / Negative staining sample screening on Talos L120C
Presentation: TBA (Takeshi Yokoyama) G3:  Negative staining sample screening on Talos L120C / Cryo sample screening on Talos Arctica
   
Day 4  Thursday, February 6th  
Open Lecture & Presenation @ Lab1 C503 Hands-on (Pre-registered members only)
Presentation: TBA (Yoshimasa Takizawa) All Groups: Relion Image Processing #1 / #2
Lecture: Advanced techniques of cryo-EM (Radostin Danev)  
Presentation: TBA (Yoshiyuki Fukuda)  
   
Day 5  Friday, February 7th  
Open Presentation @ Lab1 B503 Hands-on (Pre-registered members only)
Presentation: TBA (Kanno & Hall from Imaging Section, OIST) All Groups: Relion Image Processing #3 / #4
   
   
   

Registration: https://groups.oist.jp/img/registration-form-

We have stopped accepting applications for cryo-electron microscopy course.

*Registration is required for Hands-on session ONLY*

 

*Registration is required for Hands-on session ONLY*

*We will contact you by the beginning of January if you proceed to participate in our course*

Contact: Mizuki Tokashiki, OIST Imaging Section (mizuki.tokashiki@oist.jp)

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