In’s and Out’s of Neuronal Computation: Simultaneous Dendritic Voltage and Calcium Imaging and Somatic Recording from Purkinje Neurons in Awake Mice.

Internal Seminar : Chris Roome, Postdoctoral Researcher, Optical Neuroimaging Unit (Bernd Kuhn)

Title : In’s and Out’s of Neuronal Computation: Simultaneous Dendritic Voltage and Calcium Imaging and Somatic Recording from Purkinje Neurons in Awake Mice.

Abstract :  
Describing input-output computations of single neurons is central for understanding how information is processed within the brain. A complete explanation calls for multidimensional recording of dendritic input and somatic output from a single neuron of an awake animal. Single neuronal recordings made in vivo are however technically challenging and typically limited to anaesthetised animals, or are restricted to recording either dendritic (input) or somatic (output) activity, but not simultaneously.
Thus insofar as a description towards how dendritic inputs relate to somatic output, paired patch clamp recordings from neurons in brain slices have remained most insightful. Such preparations however lack physiological inputs present in an awake animal and are solely limited to cellular locations accessible via patch pipette. We have overcome these limitations by combining simultaneous dendritic voltage and calcium imaging with electrical somatic recording, to explore spontaneous and behavioral triggered input-output relations from single cerebellar Purkinje neurons in awake mice.
To record dendritic voltage and calcium, Purkinje neurons were labelled with voltage sensitive dye ANNINE-6plus and the genetically encoded calcium indicator GCaMP6f, using a chronic cranial window with access port (Roome and Kuhn, 2014) and imaged by two photon microscopy (line scan at 2 kHz). Somatic activity of the labelled Purkinje neurons was simultaneously recorded using an extracellular recording electrode.
Our method, permitting multidimensional recording in both temporal and spatial dimensions, provides so far the most complete description of input-output relations in Purkinje neurons in awake animals.

Refreshments will be served afterwards. We hope to see you there!

Sincerely,

Internal Seminar Organizing Committee:

Bianca Sieveritz

​ Rob Campbell

Maggi Mars-Brisbin

Yunhui Zheng

Jonathan Ward

Lauren Dembeck

Jigyasa Arora

Maéva Techer