OIST x Juntendo Joint Workshop of Tokuyasu Method 2019
Prof. Masato Koike, Juntendo University, Tokyo
Dr. Bruno M. Humbel, Okinawa Institute of Science and Technology
Venue : Okinawa Institute of Science and Technology
The Tokuyasu cryo-sectioning method is a well-established technique in Cell Biology for the localisation of macromoleculs by immuno-gold labelling. We will teach the essentials like chemical fixation and its impact on the cellular ultrastructure, how to judge the quality of antibodies and to do immunolabelling. The delegates will gain some experience in cryo-sectioning, however, need to practice in their own lab to get the necessary skills.
Interested delegates should apply with a short description of their current project and motivation to attend the course. The lectures are open to anyone but the practical part is limited to 12 participants.
- Dr. Heinz Schwarz, Max-Planck-Institute for Developmental Biology, Tübingen, Germany (Immunolabelling and Markers)
- Prof. Roland F. Fleck, King’s College, London, UK (Cryo-Preparations and Cryo-SEM)
- Prof. Masato Koike, Juntendo University Tokyo, Japan (Basic Sample Preparation for Tokuyasu Cryo-Sectioning, An Example of Application for Visualising Autophagy)
- Dr. Bruno M. Humbel, Okinawa Institute of Science and Technology, Onna-son, Japan (Chemical Fixation, Correlative Microscopy)
Participation fee: free
*Delegates who only want to attend the lectures also need to apply as the seats in the lecture theatre are limited.*
Contact: Mizuki Tokashiki, OIST Imaging Section (firstname.lastname@example.org)