Cryo-Electron Microscopy Course at OIST

Date

Monday, February 18, 2019 - 09:00 to Friday, February 22, 2019 - 18:00

Location

Lab 1 B503, Lab 3 C700 and OIST EM facility

Description

Organizer :  Bruno M. Humbel, Okinawa Institute of Science and Technology

Venue : Okinawa Institute of Science and Technology

Aim :

This course on cryo-electron microscopy is aimed at beginners. Experienced researchers will provide an overview of the current state of the art of this Nobel Prize winning technologies and share their experiences in demonstrations and hands-on practice.

It is expected that the delegates familiarised themselves with the on-line training program of ThermoFisher: https://em-learning.com/

 

Invited Instructors

Takeshi Yokoyama (RIKEN Yokohama) Yoshiyuki Fukuda (the University of Tokyo) Radostin Danev (the University of Tokyo)
Kazuyoshi Murata (NIPS) Yukihiko Sugita (Osaka University) Yoshimasa Takizawa (the University of Tokyo)
Hideki Shigematsu (RIKEN SPring-8 Center) Takayuki Kato (Osaka University)  

Keynote Lecture : Seitaro Mutoh   Former Executive Officer, Drug Discovery Research, Astellas Pharma Inc. and Professor of Kyoto Sangyo University

Opening Talk : Matthias Wolf, Okinawa Institute of Science and Technology

Schedule Morning session Afternoon Session
Day 1 : 2/18(Mon)

Open Lecture + Presentation @B503

Lecture: Sample preparation (Takeshi Yokoyama)
Presentation: Cryo-EM in NIPS Okazaki JAPAN (Kazuyoshi Murata)

Hands-on (Pre-registered members only)

G1: Negative sample preparation / Basic training on Talos L120C
G2: Cryo sample preparation / Negative sample preparation
G3: Basic training on Talos L120C / Cryo sample preparation
Day 2 : 2/19(Tue) Hands-on (Pre-registered members only)
G1: Cryo sample preparation
G2: Basic training on Talos L120C
G3: Negative sample preparation
Keynote lecture + Presentation @C700

Keynote lecture:  Evolution of drug discovery and development (Seitaro Mutoh)
 

Presentation: Cryo-electron microscopy of ribosomal complexes(Takeshi Yokoyama)

 Presenation:  In situ visual proteomics of macromoleculer complexes (Yoshiyuki Fukuda)
Day 3 : 2/20(Wed)  Open Lecture + Presentation @B503
Lecture: Electron optics and image formation (Radostin Danev)
Presenation: Cryo-EM at OIST (Yukihiko Sugita)
Hands-on (Pre-registered members only)
G1: Negative screening on Talos L120C / Relion image processing #1
G2: Cryo data acquisition on Arctica / Negative screening on Talos L120C
G3:  Relion image processing #1 / Cryo data acquisition on Arctica
Day 4 : 2/21(Thu)  Open Lecture + Presentation @B503
Lecture: Hardware;Cold-FEG, Phase Plate, Energy filter, Camera etc. (Takayuki Kato)

Lecture: Image reconstruction: principle and practice (Hideki Shigematsu)

Guest talk: Michael Elbaum from Weizmann Institute of Science
Hands-on (Pre-registered members only)
G1: Cryo data acquisition on Arctica / Relion image processing #2
G2: Relion image processing #1 / Cryo data acquisition on Krios
G3: Negative screening on Talos L120C / Relion image processing #3
Day 5 : 2/22(Fri) Open Lecture + Presentation @B503
Presentation: Challenges for the chromatin structure determination by single particle cryo-EM (Yoshimasa Takizawa)
Hands-on (Pre-registered members only)
G1: Relion image processing #3
G2: Relion image processing #2
G3: Cryo data acquisition on Krios
Hands-on (Pre-registered members only)
G1: Cryo data acquisition on Krios
G2: Relion image processing #3
G3: Relion image processing #2

Registration: https://groups.oist.jp/img/registration-form-cryo-electron-course-oist     We have stopped accepting applications for cryo-electron microscopy course.

*Registration is required for Hands-on session ONLY*

*We will contact you by the middle of January if you proceed to participate in our course*

Contact: Mizuki Tokashiki, OIST Imaging Section (mizuki.tokashiki@oist.jp)

Intra-Group Category

workshop

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