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Original Papers

2020

101 I. Koyama-Honda, T. K. Fujiwara, R. S. Kasai, K. G. N. Suzuki, E. Kajikawa, H. Tsuboi, T. A. Tsunoyama, and A. Kusumi. High-speed single-molecule imaging reveals signal transduction by induced transbilayer raft phases. J. Cell Biol. (2020). doi: 10.1083/jcb.202006125
100 P. Sil, N. Mateos, S. Nath, S. Buschow, C. Manzo, K. G. N. Suzuki, T. K. Fujiwara, A. Kusumi, M. F. Garcia-Parajo, and S. Mayor. Dynamic actin-mediated nano-scale clustering of CD44 regulates its meso-scale organization at the plasma membrane. Mol. Biol. Cell 31, 561-579 (2020). doi: 10.1091/mbc.E18-11-0715

2019

99 J. Morise, K. G. N. Suzuki, A. Kitagawa, Y. Wakazono, K. Takamiya, T. A. Tsunoyama, Y. L. Nemoto, H. Takematsu, A. Kusumi (Co-Corresponding author), and S. Oka. AMPA receptors in the synapse turnover by monomer diffusion. Nat. Commun. 10:5245 (2019). doi.org: 10.1038/s41467-019-13229-8
98 T. Matsu-Ura, H. Shirakawa, K. G. N. Suzuki, A. Miyamoto, K. Sugiura, T. Michikawa, A. Kusumi, and K. Mikoshiba. Dual-FRET imaging of IP3 and Ca2+ revealed Ca2+-induced IP3 production maintains long lasting Ca2+ oscillations in fertilized mouse eggs. Sci Rep. 9:4829 (2019). doi: 10.1038/s41598-019-40931-w

2018

97 T. A. Tsunoyama, Y. Watanabe, J. Goto, K. Naito, K. G. N. Suzuki, T. K. Fujiwara, and A. Kusumi. Super-long single-molecule tracking reveals dynamic-anchorage-induced integrin function. Nat. Chem. Biol. 14, 497-506 (2018). doi: 10.1038/s41589-018-0032-5
96 R. S. Kasai, S. V. Ito, R. M. Awane, T. K. Fujiwara, and A. Kusumi. The class-A GPCR dopamine D2 receptor forms transient dimers stabilized by agonists: detection by single-molecule tracking. Cell Biochem. Biophys. 76, 29-37 (2018). doi: 10.1007/s12013-017-0829-y
95 Y. M. Shirai, T. A. Tsunoyama, N. Hiramoto-Yamaki, K. M. Hirosawa, A. C. E. Shibata, K. Kondo, A. Tsurumune, F. Ishidate, A. Kusumi (Co-Corresponding author), and T. K. Fujiwara. Cortical actin nodes: Their dynamics and recruitment of podosomal proteins as revealed by super-resolution and single-molecule microscopy.  PLoS ONE 12, e0188778 (2018). doi: 10.1371/journal.pone.0188778

2017

94 Y. L. Nemoto, R. J. Morris, H. Hijikata, T. A. Tsunoyama, A. C. E. Shibata, R. S. Kasai, A. Kusumi (Co-Corresponding author), and T. K. Fujiwara. Dynamic meso-scale anchorage of GPI-anchored receptors in the plasma membrane: prion protein vs. Thy1. Cell Biochem. Biophys. 75, 399-412 (2017). doi: 10.1007/s12013-017-0808-3
93 S. Wakayama, S. Kiyonaka, I. Arai, W. Kakegawa, S. Matsuda, K. Ibata, Y. L. Nemoto, A. Kusumi, M. Yuzaki, and I. Hamachi. Chemical labeling for visualizing native AMPA 1 receptors in live neurons. Nat. Commun. 8, 14850 (2017). doi: 10.1038/ncomms14850 
92 M. Kinoshita, K. G. N. Suzuki, N. Matsumori, M. Takada, H. Ano, K. Morigaki, M. Abe, A. Makino, T. Kobayashi, K. M. Hirosawa, T. K. Fujiwara, A. Kusumi (Co-Corresponding author), and M. Murata. Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs. J. Cell Biol. 216, 1183-1204 (2017). doi: 10.1083/jcb.201607086
91 A. Makino, M. Abe, R. Ishitsuka, M. Murate, T. Kishimoto, S. Sakai, F. Hulin-Matsuda, Y. Shimada, T. Inaba, H. Miyatake, H. Tanaka, A. Kurahashi, C.-G. Pack, R. Kasai, S. Kubo, N. L. Schieber, N. Dohmae, N. Tochio, K. Hagiwara, Y. Sasaki, Y. Aida, F. Fujimori, T. Kigawa, K. Nishibori, R. G. Parton, A. Kusumi, Y. Sako, G. Anderluh, M. Yamashita, T. Kobayashi, P. Greimel, and T. Kobayashi. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Nieman-Pick type C. FASEB J. 31, 1301-1322 (2017). doi: 10.1096/fj.201500075R

2016

90 T. Yamada, H. Yoshimura, R. Shimada, M. Hattori, M. Eguchi, T. K. Fujiwara, A. Kusumi, T. Ozawa. Spatiotemporal analysis with a genetically encoded fluorescent RNA probe reveals TERRA function around telomeres. Sci Rep. 6:38910 (2016). doi: 10.1038/srep38910 
89 N. Komura, K. G. N. Suzuki, H. Ando, M. Konishi, M. Koikeda, A. Imamura, R. Chadda, T. K. Fujiwara, H. Tsuboi, R. Sheng, W. Cho, K. Furukawa, K. Furukawa, Y. Yamauchi, H. Ishida, A. Kusumi (Co-Corresponding Author), and M. Kiso. Raft-based interactions of gangliosides with a GPI-anchored receptor. Nat. Chem. Biol. 12, 402-410 (2016). doi: 10.1038/nchembio.2059 
88 T. K. Fujiwara, K. Iwasawa, Z. Kalay, T. A. Tsunoyama, Y. Watanabe, Y. M. Umemura, H. Murakoshi, K. G. N. Suzuki, Y. L. Nemoto, N. Morone, and A. Kusumi. Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane. Mol. Biol. Cell 27, 1101-1119 (2016). doi: 10.1091/mbc.E15-04-0186

2014

87 Z. Kalay, T. K. Fujiwara, A. Otaka, and A. Kusumi. Lateral diffusion in a discrete fluid membrane with immobile particles. Phys. Rev. E 89, 022724 (2014). doi: 10.1103/PhysRevE.89.022724
86 N. Hiramoto-Yamaki, K. A. K. Tanaka, K. G. N. Suzuki, M. S.H. Miyahara, Z. Kalay, K. Tanaka, R. S. Kasai, and A. Kusumi (co-corresponding author), and T. K. Fujiwara. Ultrafast diffusion of a fluorescent cholesterol analog in compartmentalized plasma membranes. Traffic 15, 583-612 (2014). doi: 10.1111/tra.12163

2013

85 N. Yoshikawa, H. Hirori, H. Watanabe, T. Aoki, T. Ihara, R. Kusuda, C. Wolpert, T. K. Fujiwara, A. Kusumi, Y. Kanemitsu, and K. Tanaka. Biexciton state causes photoluminescence fluctuations in CdSe/ZnS core/shell quantum dots at high photoexcitation densities. Phys. Rev. B 88, 155440, 1-5 (2013). doi: 10.1103/PhysRevB.88.155440
84 H. Nishimura, K. Ritchie, R. S. Kasai, N. Morone, H. Sugimura, K. Tanaka, I. Sase, A. Yoshimura, Y. Nakano, T. K. Fujiwara, and A. Kusumi. Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging. J. Cell Biol. 202, 967-983 (2013). (Featured in “In This Issue” of the journal) doi: 10.1083/jcb.201301053​
83 K. O. Nagata, C. Nakada, R. S. Kasai, and A. Kusumi (co-corresponding author) and K. Ueda. ABCA1 dimer-monomer interconversion during HDL generation revealed by single-molecule imaging. Proc. Natl. Acad. Sci. USA 110, 5034-5039 (2013). doi: 10.1073/pnas.1220703110 
82

A. C. E. Shibata, L.-M. H. Chen, R. Nagai, F. Ishidate, R. Chadda, Y. Miwa, K. Naruse, Y. M. Shirai, T. K. Fujiwara, and A. Kusumi. Rac1 recruitment to the archipelago structure of the focal adhesion through the fluid membrane as revealed by single-molecule analysis. Cytoskeleton 70, 161-177 (2013). (Adopted as the cover page figure) doi: 10.1002/cm.21097

2012

81 R. Sheng, Y. Chen, H. Y. Gee, E. Stec, H. R. Melowic, N. R. Blatner, M. P. Tun, Y. Kim, M. Källberg, T. K. Fujiwara, J. H. Hong, K. P. Kim, H. Lu, A. Kusumi, M. G. Lee, and W. Cho. Cholesterol modulates cell signaling and protein networking by specifically interacting with PDZ domain-containing scaffold proteins. Nat. Commun. 3:1249 (2012). doi: 10.1038/ncomms2221
80 K. G. N. Suzuki, R. S. Kasai, K. M. Hirosawa, Y. L. Nemoto, M. Ishibashi, Y. Miwa, T. K. Fujiwara, and A. Kusumi. Transient GPI-anchored protein homodimers are units for raft organization and function. Nat. Chem. Biol. 8, 774-783 (2012). (Featured in “News and Views” of the journal; Selected as one of the 10-year greatest hits of this journal) doi: 10.1038/nchembio.1028
79

A. C. E. Shibata, T. K. Fujiwara, L.-M. H. Chen, K. G. N. Suzuki, Y. Ishikawa, Y. L. Nemoto, Y. Miwa, R. Chadda, K. Naruse, and A. Kusumi. Archipelago architecture of the focal adhesion: Membrane molecules freely enter and exit from the focal adhesion zone. Cytoskeleton 69, 380-392 (2012). (Adopted as the cover page figure) doi: 10.1002/cm.21032​

78 K.-J. Cho, R. S. Kasai, J.-H. Park, S. Chigurupati, S. J. Heidorn, D. van der Hoeven, S. J. Plowman, A. Kusumi, R. Marais, and J. F. Hancock. Raf inhibitors target Ras spatiotemporal dynamics. Curr. Biol. 22, 945-955 (2012).  dio: 10.1016/j.cub.2012.03.067
77 Z. Kalay, T. Fujiwara, and A. Kusumi. Confining domains lead to reaction bursts: reaction kinetics in the plasma membrane. PLoS One 7, e32948 (2012).  doi: 10.1371/journal.pone.0032948

2011

76 A. Hagiwara, Y. Tanaka, R. Hikawa, N. Morone, A. Kusumi, H. Kimura, and M. Kinoshita. Submembranous septins as relatively stable components of actin-based membrane skeleton. Cytoskeleton 68, 512-525 (2011). doi: 10.1002/cm.20528
75 X. Li, L. Liu, L. Wang, K. Kamei, Q. Yuan, F. Zhang, J. Shi, A. Kusumi, M. Xie, Z. Zhao, and Y. Chen. Integrated and diffusion-based micro-injectors for open access cell assays. Lab Chip 11, 2612-2617 (2011). doi: 10.1039/C1LC20258H
74 R. S. Kasai, K. G. N. Suzuki, E. R. Prossnitz, I. Koyama-Honda, C. Nakada, T. K. Fujiwara, and A. Kusumi. Full characterization of GPCR monomer-dimer dynamic equilibrium by single molecule imaging. J. Cell Biol. 192, 463-480 (2011). doi: 10.1083/jcb.201009128

2010

73 K. A. K. Tanaka, K. G, N. Suzuki, Y. M. Shirai, S. T. Shibutani, M. S. H. Miyahara, H. Tsuboi, M. Yahara, A. Yoshimura, S. Mayor, T. K. Fujiwara, and A. Kusumi. Membrane molecules mobile even after chemical fixation. Nat. Methods 7, 865-866 (2010). doi: 10.1038/nmeth.f.314

2008

72 Y. M. Umemura, M. Vrljic, S. Y. Nishimura, T. K. Fujiwara, K. G. N. Suzuki, and A. Kusumi. Both MHC classII and its GPI-anchored form undergo hop diffusion as observed by single-molecule tracking. Biophys. J. 95, 435-450 (2008).  doi: 10.1529/biophysj.107.123018

2007

71 K. G. N. Suzuki, T. K., Fujiwara, M. Edidin, and A. Kusumi. Dynamic recruitment of phospholipase Cγ at transiently immobilized GPI-anchored receptor clusters induces IP₃-Ca2+ signaling: single-molecule tracking study 2. J. Cell Biol. 177, 731-742 (2007). doi: 10.1083/jcb.200609175 
70 K. G. N. Suzuki, T. K., Fujiwara, F. Sanematsu, R. Iino, M. Edidin, and A. Kusumi. GPI-anchored receptor clusters transiently recruit Lyn and Gα for temporary cluster immobilization and Lyn activation: single-molecule tracking study 1. J. Cell Biol. 177, 717-730 (2007). doi: 10.1083/jcb.200609174
69 W. K. Subczynski, A. Wisniewska, J. S. Hyde, and A. Kusumi. Three-dimensional dynamic structure of the liquid-ordered domain in lipid membranes as examined by pulse-EPR oxygen probing. Biophys. J. 92, 1573-1584 (2007). doi:10.1529/biophysj.106.097568

2006

68

N. Morone, T. Fujiwara, K. Murase, R. S. Kasai, H. Ike, S. Yuasa, J. Usukura, and A. Kusumi. Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography. J. Cell Biol. 174, 851-62 (2006). (Featured in “In This Issue” of the journal: Adopted as the cover page figure) doi: 10.1083/jcb.200606007

2005

67 W. K. Subczynski, A. Wisniewska, A. Kusumi, and R. N. McElhaney. Effects of pH-induced variations of the charge of the transmembrane α-helical peptide Ac-K2(LA)12K2-amide on the organization and dynamics of the host dimyristoylphosphatidylcholine bilayer membrane. Biochim. Biophys. Acta – Biomembranes 1720, 99-109 (2005). doi: 10.1016/j.bbamem.2005.11.014
66 K. Suzuki, K. Ritchie, E. Kajikawa, T. Fujiwara, and A. Kusumi. Rapid hop diffusion of a G-protein-coupled receptor in the plasma membrane as revealed by single-molecule techniques. Biophys. J. 88, 3659-3680 (2005) doi: 10.1529/biophysj.104.048538
65 I. Koyama-Honda, K. Ritchie, T. Fujiwara, R. Iino, H. Murakoshi, R. S. Kasai, and A. Kusumi. Fluorescence imaging for monitoring the colocalization of two single molecules in living cells. Biophys. J. 88, 2126-2136 (2005). doi: 10.1529/biophysj.104.048967
64 K. Ritchie, X.-Y. Shan, J. Kondo, K. Iwasawa, T. Fujiwara, and A. Kusumi. Detection of non-Brownian diffusion in the cell membrane in single molecule tracking. Biophys. J. 88, 2266-2277 (2005). doi: 10.1529/biophysj.104.054106

2004

63 T. Rog, K. Murzyn, R. Grubiel, Y. Takaoka, A. Kusumi, and M. Pasenkiewicz-Gierula. Effects of phospholipid unsaturation on the bilayer nonpolar region: a molecular simulation study. J. Lipid Res. 45, 326-336 (2004). doi: 10.1194/jlr.M300187-JLR200
62 K. Murase, T. Fujiwara, Y. Umemura, K. Suzuki, R. Iino, H. Yamashita, M. Saito, H. Murakoshi, K. Ritchie, and A. Kusumi. Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques. Biophys. J. 86, 4075-4093 (2004). doi: 10.1529/biophysj.103.035717
61 H. Murakoshi, R. Iino, T. Kobayashi, T. Fujiwara, C. Ohshima, A. Yoshimura, and A. Kusumi. Single-molecule imaging analysis of Ras activation in living cells. Proc. Natl. Acad. Sci. U.S. A. 101, 7317-7322 (2004). doi: 10.1073/pnas.0401354101

2003

60 C. Nakada, K. Ritchie, Y. Oba, M. Nakamura, Y. Hotta, R. Iino, R., R. S. Kasai, K. Yamaguchi, T. Fujiwara, and A. Kusumi. Accumulation of anchored proteins forms membrane diffusion barriers as neurons develop polarization. Nat. Cell Biol. 5, 626-632  (Featured in “News and Views”) (2003). doi: 10.1038/ncb1009
59 H. Ike, A. Kosugi, A. Kato, R. Iino, H. Hirano, T. Fujiwara, K. Ritchie, and A. Kusumi. Mechanism of Lck recruitment to the T-cell receptor cluster as studied by single molecule fluorescence video imaging. ChemPhysChem 4, 620-626 (2003). doi: 10.1002/cphc.200300670
58 W. K. Subczynski, M. Pasenkiewicz-Gierula, R. N. McElhaney, J. S. Hyde, and A. Kusumi. Molecular dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine membranes containing transmembrane α-helical peptides with alternating leucine, alanine residues. Biochemistry 42, 3939-3948 (2003). doi: 10.1021/bi020636y

2002

57 I. Dan, N. M. Watanabe, E. Kajikawa, T. Ishida, A. Pandey, and A. Kusumi. Overlapping of MINK and CHRNE gene loci in the course of mammalian evolution. Nucleic Acids Res. 30, 2906-2910 (2002). doi: 10.1093/nar/gkf407
56 K. Ritchie and A. Kusumi. Single molecule probe scanning optical force imaging microscope for viewing live cells. J. Biol. Phys. 28, 619-626 (2002). doi: 10.1023/A:1021282504628
55

T. Fujiwara, K. Ritchie, H. Murakoshi, K. Jacobson, and A. Kusumi. Phospholipids undergo hop diffusion in compartmentalized cell membrane. J. Cell Biol. 157, 1071-1081(2002). (Adopted as the cover page figure)  doi: 10.1083/jcb.200202050

54 C. Dietrich, B. Yang, T. Fujiwara, A. Kusumi, and K. Jacobson. Relationship of lipid rafts to transient confinement zones detected by single particle tracking. Biophys. J. 82, 274-284 (2002). doi: 10.1016/S0006-3495(02)75393-9

2001

53 R. N. A. H. Lewis, Y.-P. Zhang, R. S. Hodges, W. K. Subczynski, A. Kusumi, C. R. Flach, R. Mendelsohn, and R. N. McElhaney. A polyalanine-based peptide cannot form a stable transmembrane alpha-helix in fully hydrated phospholipid bilayers. Biochemistry, 40, 12103-12111(2001). doi:10.1021/bi010555m
52 S. Ohta-Iino, M. Pasenkiewicz-Gierulla, Y. Takaoka, H. Miyagawa, K. Kitamura, and A. Kusumi. Fast lipid disorientation at the onset of membrane fusion revealed by molecular dynamics simulations. Biophys. J. 81, 217-224 (2001). doi: 10.1016/S0006-3495(01)75693-7
51 R. Iino, I. Koyama, and A. Kusumi. Single molecule imaging of GFP in living cells: E-cadherin forms oligomers on the free cell surface. Biophys. J. 80, 2667-2677 (2001). doi: 10.1016/S0006-3495(01)76236-4
50 K. Kawasaki, J.-J. Yin, W. K. Subczynski, J. S. Hyde, and A. Kusumi. Pulse EPR observation of lipid exchange between protein-rich raft and bulk domains in the membrane. Biophys. J. 80, 738-748 (2001). doi: 10.1016/S0006-3495(01)76053-5

2000

49 Y. Takaoka, M. Pasenkiewicz-Gierula, H. Miyagawa, K. Kitamura, Y. Tamura, and A. Kusumi. Molecular dynamics generation of non-arbitrary membrane models reveals lipid orientational correlations. Biophys. J. 79, 3118-3138 (2000). doi: 10.1016/S0006-3495(00)76546-5
48 I. Dan, N. M. Watanabe, T. Kobayashi, K. Yamashita-Suzuki, Y. Fukagaya, E. Kajikawa, W. K. Kimura, T. M. Nakashima, K. Matsumoto, J. Ninomiya-Tsuji, and A. Kusumi. Molecular cloning of MINK, a novel member of mammalian GCK family kinases, which is upregulated during postnatal mouse cerebral development. FEBS Lett. 469, 19-23 (2000). doi: 10.1016/s0014-5793(00)01247-3
47

M. Pasenkiewicz-Gierula, T. Rog, K. Kitamura, and A. Kusumi. Cholesterol effects on the phosphatidylcholine bilayer polar region: a molecular simulation study. Biophys. J. 78, 1376-1389 (2000). (Adopted as the cover page figure)  doi: 10.1016/S0006-3495(00)76691-4

1999

46 M. Tomishige, and A. Kusumi. Compartmentalization of the erythrocyte membrane by the membrane skeleton: intercompartmental hop diffusion of band 3. Mol. Biol. Cell 10, 2475-2479 (1999). doi: 
45 M. Pasenkiewicz-Gierula, Y. Takaoka, H. Miyagawa, K. Kitamura, and A. Kusumi. Charge-pairing of headgroups in phosphatidylcholine membranes. A molecular dynamics simulation study. Biophys. J. 76, 1228-1240 (1999). doi: 10.1016/S0006-3495(99)77286-3
44 S. Toyoda, H. Miyagawa, T. Amisaki, E. Hashimoto, J. Ikeda, A. Kusumi, K. Kitamura, and N. Miyakawa. Development of MD-engine, a special purpose accelerator for molecular dynamics simulations. J. Comp. Chem. 20, 185-199 (1999). doi: 10.1002/(SICI)1096-987X(19990130)20:2<185::AID-JCC1>3.0.CO;2-L

1998

43 K. Yamaguchi, Y. Tsuda, T. Shimakage, and A. Kusumi. Synthesis of phospholipids containing 2-nitrobenzyl ester moieties at the terminals of alkyl chains and properties of photodegradable liposomes from the lipids. Bull. Chem. Soc. Japan 71, 1923-1929 (1998). doi: 10.1246/bcsj.71.1923
42 M. Tomishige, M. Takeuchi, Y. Sako, Y. Takakuwa, and A. Kusumi. Regulation mechanism of the lateral diffusion of band 3 in erythrocyte membranes by the membrane skeleton. J. Cell Biol. 142, 989-1000 (1998). doi: 10.1083/jcb.142.4.989
41 M. Takeuchi, H. Miyamoto, H. Komizu, Y. Sako, and A. Kusumi. Structure of the erythrocyte membrane skeleton as observed by atomic force microscopy. Biophys. J. 74, 2171-2183 (1998). doi: 10.1016/S0006-3495(98)77926-3
40 W. K. Subczynski, R. N. A. H. Lewis, R. N. McElhaney, R. S. Hodges, J. S. Hyde, and A Kusumi. Molecular organization and dynamics of 1-palmitoyl-2-oleoylphosphatidylcholine bilayers containing a transmembrane a-helical peptide. Biochemistry 37, 3156-3164 (1998). doi: 10.1021/bi972148+
39 Y. Sako, A. Nagafuchi, S. Tsukita, M. Takeichi, and A. Kusumi. Cytoplasmic regulation of the movements of E-cadherin in the plasma membrane as studied by optical tweezers and single particle tracking. J. Cell Biol. 140, 1227-1240 (1998). doi: 10.1083/jcb.140.5.1227

1997

38 M. Pasenkiewicz-Gierula, Y. Takaoka, H. Miyagawa, K. Kitamura, and A. Kusumi. Hydrogen bonding of water to phosphatidylcholine in the membrane as studied by a molecular dynamics simulation: location, geometry, and lipid-lipid bridging via hydrogen-bonded water. J. Phys. Chem. 101, 3677-3691 (1997). doi: 10.1021/jp962099v
37 Y. Sako, A. Sekihata, Y. Yanagisawa, M. Yamamoto, Y. Shimada, K. Ozaki, and A. Kusumi. Comparison of two-photon excitation laser scanning fluorescence microscopy with UV-confocal laser scanning fluorescence microscopy. J. Microscopy 185, 9-20 (1997). doi: 10.1046/j.1365-2818.1997.1480707.x

1996

36 H. Muramatsu, N. Chiba, T. Umemoto, K. Homma, K. Nakajima, T. Ataka, S. Ohta, A. Kusumi, and M. Fujihira. Development of near-field optic/atomic-force microscope for biological materials in aqueous solutions. Ultramicroscopy 61, 265-269 (1996). doi: 10.1016/0304-3991(95)00113-1
35 H. Muramatsu, N. Chiba, K. Homma, K. Nakajima, T. Ataka, S. Ohta, A. Kusumi, and M. Fujihira. Scanning near-field optic/atomic force microscopy in liquids. Thin Solid Films 273, 337-338 (1996). doi: 10.1016/0040-6090(95)06798-1
34 A. Wisniewska, Y. Nishimoto, J. S. Hyde, A. Kusumi, and W. K. Subczynski. Depth dependence of the perturbing effect of placing a bulky group (oxazolidine ring spin labels) in the membrane on the membrane phase transition. Biochim. Biophys. Acta 1278, 68-72 (1996). doi: 10.1016/0005-2736(96)89535-6

1995

33 H. Muramatsu, N. Chiba, K. Homma, K. Nakajima, T. Ataka, S. Ohta, A. Kusumi, and M. Fujihira. Near-field optical microscopy in liquids. Appl. Phys. Lett. 66, 3245-3247 (1995). doi: 10.1063/1.113392
32 Y. Sako and A. Kusumi. Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether. J. Cell Biol. 129, 1559-1574 (1995). doi: 10.1083/jcb.129.6.1559
31 T. Amisaki, T. Fujiwara, A. Kusumi, H. Miyagawa, and K. Kitamura. Error evaluation in the design of a special-purpose processor that calculates non-bonded forces in molecular dynamics simulations. J. Comp. Chem. 16, 1120-1130 (1995). doi: 10.1002/jcc.540160906

1994

30 W. K. Subczynski, A. Wisniewska, J.-J. Yin, J. S. Hyde, and A. Kusumi. Hydrophobic barriers of lipid bilayer membranes formed by reduction of water penetration by alkyl chain unsaturation and cholesterol. Biochemistry 33, 7670-7681 (1994). doi: 10.1021/bi00190a022
29 Y. Sako, and A. Kusumi. Compartmentalized structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis. J. Cell Biol. 125, 1251-1264 (1994). doi: 10.1083/jcb.125.6.1251
28 I. Ashikawa, J. Yin, W. K. Subczynski, T. Kouyama, J. S. Hyde, and A. Kusumi. Molecular organization and dynamics in bacteriorhodopsin-rich reconstituted membranes; Discrimination of lipid environments by the oxygen transport parameter using a pulse ESR spin-labeling technique. Biochemistry 33, 4947-4952 (1994). doi: 10.1021/bi00182a025

1993

27 A. Kusumi, Y. Sako, and M. Yamamoto. Confined lateral diffusion of membrane receptors as studied by single particle tracking (nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells. Biophys. J. 65, 2021-2040 (1993). doi: 10.1016/S0006-3495(93)81253-0 
26 T. Oida, Y. Sako, and A. Kusumi. Fluorescence lifetime imaging microscopy (flimscopy): Methodology development and its application to studies of endosome fusion in single cells. Biophys. J. 64, 676-685 (1993). doi: 10.1016/S0006-3495(93)81427-9

1992

25 W. K. Subczynski, G. E. Renk, R. K. Crouch, J. S. Hyde, and A. Kusumi. Oxygen diffusion-concentration product in rhodopsin as observed by a pulse ESR spin labeling method. Biophys. J. 63, 573-577 (1992). doi: 10.1016/S0006-3495(92)81612-0

1991

24 W. K. Subczynski, J. S. Hyde, and A. Kusumi. Effect of alkyl chain unsaturation and cholesterol intercalation on oxygen transport in membranes: a pulse ESR spin labeling study. Biochemistry 30, 8578-8590 (1991). doi: 10.1021/bi00099a013
23 A. Kusumi, A. Tsuji, M. Murata, Y. Sako, A. Yoshizawa, T. Hayakawa, and S. Ohnishi. Development of a streak camera-based time-resolved microscope fluorimeter and its application to studies of membrane fusion in single cells. Biochemistry 30, 6517-6527 (1991). doi: 10.1021/bi00240a024

1990

22 W. K. Subczynski, W. E. Antholine, J. S. Hyde, and A. Kusumi. Microimmiscibility and three- dimensional structure of unsaturated phosphatidylcholine-cholesterol membranes: translational diffusion of a square-planar copper complex in the membrane. Biochemistry 29, 7936-7945 (1990). doi: 10.1021/bi00486a023
21 M. Pasenkiewicz-Gierula, W. K. Subczynski, and A. Kusumi. Rotational diffusion of a steroid molecule in phosphatidylcholine-cholesterol membranes: fluid phase microimmiscibility in unsaturated-phosphatidylcholine-cholesterol membranes. Biochemistry 29, 4059-4069 (1990). doi: 10.1021/bi00469a006

1989

20 W. K. Subczynski, J. S. Hyde, and A. Kusumi. Oxygen permeability of phosphatidylcholine-cholesterol membranes. Proc. Natl. Acad. Sci. USA 86, 4474-4478 (1989). doi: 10.1073/pnas.86.12.4474
19 A. Kusumi, S. Nakahama, and K. Yamaguchi. Liposomes that can be disintegrated by photo-irradiation. Chem. Lett. 433-436 (1989). doi: 10.1246/cl.1989.433

1988

18 A. Tsuji, K. Kawasaki, S. Ohnishi, H. Merkle, and A. Kusumi. Regulation of band 3 mobilities in erythrocyte ghost membranes by protein association and cytoskeletal meshwork. Biochemistry 27, 7447-7452 (1988). doi: 10.1021/bi00419a041
17 A. Kusumi and M. Pasenkiewicz-Gierula. Rotational diffusion of a steroid molecule in phosphatidylcholine membranes: Effects of alkyl chain length, unsaturation and cholesterol as studied by a spin label method. Biochemistry 27, 4407-4418 (1988). doi: 10.1021/bi00412a030

1987

16 H. Merkle, W. K. Subczynski, and A. Kusumi. Dynamic fluorescence quenching studies on lipid mobilities in phosphatidylcholine-cholesterol membranes. Biochim. Biophys. Acta 897, 238-248 (1987). doi: 10.1016/0005-2736(87)90420-2

1986

15 A. Kusumi and J. L. Winkelhake. Fc:Fc interactions revealed by spin-labeled IgG heterosaccharides in model immune complexes. Biochem. Biophys. Res. Commun. 137, 237- 243 (1986). doi: 10.1016/0006-291x(86)91201-5
14 W. K. Subczynski and A. Kusumi. Effects of very small amounts of cholesterol on gel-phase membranes: A spin label study. Biochim. Biophys. Acta 854, 318-320 (1986). doi: 10.1016/0005-2736(86)90125-2
13 A. Kusumi, W. K. Subczynski, M. Pasenkiewicz-Gierula, J. S. Hyde, and H. Merkle. Spin-label studies on phosphatidylcholine-cholesterol membranes: Effects of alkyl chain length and unsaturation in the fluid phase. Biochim. Biophys. Acta 854, 307-317 (1986). doi: 10.1016/0005-2736(86)90124-0

1985

12 W. K. Subczynski and A. Kusumi. Detection of oxygen consumption during very early stages of lipid peroxidation by ESR nitroxide probe method. Biochim. Biophys. Acta 821, 259-263 (1985). doi: 10.1016/0005-2736(85)90095-1

1984

11 L. Koulu, A. Kusumi, M. S. Steinberg, V. Klaus-Kovtun, and J. R. Stanley. Human autoantibodies against a core desmosomal protein in pemphigus foliaceus. J. Exp. Med. 160, 1509-1518  (1984). doi: 10.1084/jem.160.5.1509
10 C. J. Taves, A. Kusumi, and J. L. Winkelhake. Human aglycosyl-IgG exhibits increased hydrophobicity: Binding/fluorescence studies with 8-anilinonaphthalene-1-sulfonic acid (ANS). Biochem. Biophys. Res. Commun. 124, 605-613 (1984). doi: 10.1016/0006-291x(84)91597-3
9 J. L. Winkelhake, A. Kusumi, L. McKean, and W. J. Mandy. Complement C1q binding affects spin-labeled heterosaccharides of rabbit antibodies in immune not artificial IgG aggregates. J. Biol. Chem. 259, 2171-2178  (1984). doi: 10.1016/S0021-9258(17)43333-3

1983

8 A. Kusumi, M. Singh, D. A. Tirrell, G. Oehme, A. Singh, N. K. P. Samuel, J. S. Hyde, and S. L. Regen. Dynamic and structural properties of polymerized phosphatidylcholine vesicle membranes. J. Am. Chem. Soc. 105, 2975-2980  (1983). doi: 10.1021/ja00348a009
7 A. Kusumi, M. Tsuda, T. Akino, S. Ohnishi, and Y. Terayama. Protein-phospholipid-cholesterol interaction in the photolysis of invertebrate rhodopsin. Biochemistry 22, 1165-1170 (1983). doi: 10.1021/bi00274a027

1982

6 J. S. Hyde, W. Froncisz, and A. Kusumi. Dispersion ESR with the loop-gap resonator. Rev. Sci. Instr. 53, 1934-1937 (1982). doi: 10.1063/1.1136918
5 A. Kusumi and J. S. Hyde. Spin-label saturation-transfer electron spin resonance detection of transient association of rhodopsin in reconstituted membranes. Biochemistry 21, 5978-5983  (1982). doi: 10.1021/bi00266a039
4 A. Kusumi, W. K. Subczynski, and J. S. Hyde. Oxygen transport parameter in membranes as deduced by saturation recovery measurements of spin-lattice relaxation times of spin labels. Proc. Natl. Acad. Sci. USA 77, 1854-1858  (1982). doi: 10.1073/pnas.79.6.1854

1980

3 A. Kusumi, S. Ohnishi, and M. Tsuda. Light-induced conformational change of octopus rhodopsin as detected by a spin-label method. Biochem. Biophys. Res. Commun. 95, 1635-1641  (1980). doi: 10.1016/S0006-291X(80)80086-6
2 A. Kusumi, T. Sakaki, T. Yoshizawa, and S. Ohnishi. Protein-lipid interactions in rhodopsin recombinant membranes as studied by protein rotational mobility and lipid alkyl chain flexibility measurements. J. Biochem. 88, 1103-1111 (1980). doi: 10.1093/oxfordjournals.jbchem.a133063

1978

1 A. Kusumi, S. Ohnishi, T. Ito, and T. Yoshizawa. Rotational motion of rhodopsin in the visual receptor membrane as studied by saturation transfer spectroscopy. Biochim. Biophys. Acta 507, 539-543 (1978). doi: 10.1016/0005-2736(78)90362-0

 


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Review Articles

2020

37 A. Kusumi, T. K. Fujiwara, T. A. Tsunoyama, R. S. Kasai, A.-A. Liu, K. M. Hirosawa, M. Kinoshita, M. Matsumori, N. Komura, H. Ando, and K. G. N. Suzuki. Defining raft domains in the plasma membrane. Traffic 21, 106-137 (2020). (20th Anniversary Issue of Traffic) doi: 10.1111/tra.12718

2018

36 K. G. N. Suzuki, H. Ando, N. Komura, K. Fujiwara, M. Kiso, and A. Kusumi. Unraveling of lipid raft organization in cell plasma membranes by single-molecule imaging of ganglioside probes. Adv. Exp. Med. Biol. 1104, 41-58 (2018).  doi: 10.1007/978-981-13-2158-0_3
35 K. G. N. Suzuki, H. Ando, N. Komura, M. Konishi, A. Imamura, H. Ishida, M. Kiso, T. K. Fujiwara, and A. Kusumi. Revealing the raft domain organization in the plasma membrane by single-molecule imaging of fluorescent ganglioside analogs. Methods Enzymol. 598, 267-282 (2018).  doi: 10.1016/bs.mie.2017.06.038

2017

34 N. Komura, K. G. N. Suzuki, H. Ando, M. Konishi, A. Imamura, H. Ishida, A. Kusumi, and M. Kiso. Synthesis of fluorescent gangliosides for the studies of raft domains. Methods Enzymol. 597, 239-263 (2017). doi: 10.1016/bs.mie.2017.06.004
33 K. G. N. Suzuki, H. Ando, N. Komura, T. Fujiwara, M. Kiso, and A. Kusumi. Development of new ganglioside probes and unraveling of raft domain structure by single-molecule imaging. Biochim. Biophys. Acta - General Subjects (Review in a special issue on Neuro-glycoscience) 1861, 2494-2506 (2017). doi:10.1016/j.bbagen.2017.07.012

2014

32 A. Kusumi and R. S. Kasai. Class A GPCRs’ transient homodimers might be important for some of their key functions. J. Physiol. 592 Online Session “CROSSTALK” (2014).
31 A. Kusumi, T. A. Tsunoyama, K. M. Hirosawa, R. S. Kasai, and T. K. Fujiwara. Tracking single molecules at work in living cells. Nat. Chem. Biol. 10, 524-532 (2014). (Introduced in the Editorial) doi: 10.1038/nchembio.1558
30 R. S. Kasai and A. Kusumi. Single-molecule imaging revealed dynamic GPCR dimerization. Curr. Opin. Cell Biol. 27, 78-86 (2014). doi: 10.1016/j.ceb.2013.11.008

2013

29 K. G. N. Suzuki, R. S. Kasai, T. K. Fujiwara and A. Kusumi. Single-molecule imaging of receptor-receptor interactions. Methods Cell Biol. 117, 373-390 (2013). doi: 10.1016/B978-0-12-408143-7.00020-7

2012

28 A. Kusumi. An MBoC 20th Anniversary Favorite: Gangliosides GM1 and GM3 in the living cell membrane form clusters susceptible to cholesterol depletion and chilling. Mol. Biol. Cell 23, 3925 (2012). In celebration of MBoC’s first 20 years, members of the ASCB Council were asked to comment on their favorite MBoC papers from the past two decades. doi: 10.1091/mbc.e12-02-0159
27 A. Kusumi, T. K. Fujiwara, R. Chadda, M. Xie, T. A. Tsunoyama, Z. Kalay, R. S. Kasai, and K. G. N. Suzuki. Dynamic organizing principles of the plasma membrane that regulate signal transduction: Commemorating the fortieth anniversary of Singer and Nicolson’s fluid-mosaic model. Ann. Rev. Cell Dev. Biol. 28, 215-250 (2012). doi: 10.1146/annurev-cellbio-100809-151736
26 A. Kusumi, T. K. Fujiwara, N. Morone, K. J. Yoshida, R. Chadda, M. Xie, R. S. Kasai, and K. G. N. Suzuki. Membrane mechanisms for signal transduction: The coupling of the meso-scale raft domains to membrane-skeleton-induced compartments and dynamic protein complexes. Seminars Cell Develop. Biol. 23, 126-144 (2012). doi: 10.1016/j.semcdb.2012.01.018

2011

25

A. Kusumi, K. G. N. Suzuki, R. S. Kasai, K. Ritchie, and T. K. Fujiwara. Hierarchical mesoscale domain organization of the plasma membrane. Trends Biochem. Sci. 36, 604-615 (2011).  (Adopted as the cover page figure)  doi: 10.1016/j.tibs.2011.08.001

24 Z. Kalay, T. K. Fujiwara, and A. Kusumi. Cytoskeleton-induced meso-scale domains. In “Cellular Domains”, Chapter 1, I. R. Nabi Ed., Wiley pp. 3-22 (2011). doi: 10.1002/9781118015759.ch1

2010

23 A. Kusumi, Y. Shirai, I. Koyama-Honda, K. G. N. Suzuki, and T. K. Fujiwara. Hierarchical organization of the plasma membrane: investigations by single-molecule tracking vs. fluorescence correlation spectroscopy. FEBS Lett. 584, 1814-1823 (2010). doi: 10.1016/j.febslet.2010.02.047

2008

22 K. G. N. Suzuki, and A. Kusumi. Mechanism for signal transduction in the induced-raft domains as revealed by single molecule tracking. Trends in Glycoscience and Glycotechnology 20, 341-351 (2008). doi: 10.4052/tigg.20.341
21 A. Kusumi, Y. Umemura, N. Morone, and T. Fujiwara. Paradigm shift of the molecular dynamics concept in the cell membrane: high-speed single-molecule tracking revealed the partitioning of the cell membrane. In “Anomalous Transport: Foundations and Applications”, Chapter 19, R. Klages Ed., Wiley pp. 545-574 (2008). doi: 10.1002/9783527622979.ch19
20 N. Morone, C. Nakada, Y. Umemura, J. Usukura, and A. Kusumi. Three-dimensional molecular architecture of the plasma-membrane-associated cytoskeleton as reconstructed by freeze-etch electron tomography. Methods in Cell Biology 88, “Introduction to Electron Microscopy for Biologists”, Chapter 12, T. Allen Ed. 207-236 (2008). doi: 10.1016/S0091-679X(08)00412-3

2007

19 W. K. Subczynski, J. Widomska, A. Wisniewska, and A. Kusumi. Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains. Methods Mol. Biol. 398, 143-57 (2007). doi: 10.1007/978-1-59745-513-8_11

2005

18 A. Kusumi, and K. Suzuki. Toward understanding the dynamics of membrane-raft-based molecular interactions. Biochim. Biophys. Acta: Reviews on Mol. Cell Biol. 1746, 234-251 (2005). doi: 10.1016/j.bbamcr.2005.10.001
17 A. Kusumi, K. Suzuki, J. Kondo, N. Morone, and Y. Umemura. Protein-lipid interactions in the formation of raft microdomains in biological membranes. In "Protein-lipid interactions: from membrane domains to cellular networks", Chapter 13, L. Tamm, Ed. Wiley pp. 307-336 (2005). doi: 10.1002/3527606769.ch13
16 A. Kusumi, C. Nakada, K. Ritchie, K. Murase, K. Suzuki, H. Murakoshi, R. S. Kasai, J. Kondo, and T. Fujiwara. Paradigm shift of the plasma membrane concept from the two-dimensional continuum fluid to the partitioned fluid: high-speed single-molecule tracking of membrane molecules. Annu. Rev. Biophys. Biomol. Struct. 34, 351-378 (2005). doi: 10.1146/annurev.biophys.34.040204.144637
15 A. Kusumi, H. Ike, C. Nakada, K. Murase, and T. Fujiwara. Single-molecule tracking of membrane molecules: plasma membrane compartmentalization and dynamic assembly of raft-philic signaling molecules. Sem. Immunol. 17, 3-21(2005). doi: 10.1016/j.smim.2004.09.004
14 A. Kusumi, H. Murakoshi, K. Murase, and T. Fujiwara. Single-molecule imaging of diffusion, recruitment, and activation of signaling molecules in living cells. In "Biophysical aspects of transmembrane signaling", Chapter 5, S. Damjanovich, Ed. Springer-Heidelberg pp. 123-152 (2005). doi: 10.1007/3-540-26511-2_5

2004

13

A. Kusumi, I. Koyama-Honda, and K. Suzuki. Molecular dynamics and interactions for creation of signal-induced stabilized rafts from small unstable steady-state rafts. Traffic 5, 213-230 (2004). (Adopted as the cover page figure) doi: 10.1111/j.1600-0854.2004.0178.x

12 K. Ritchie, and A. Kusumi. Role of the membrane skeleton in creation of microdomains. Subcellular Biochem. 37, 233-245  (2004).  doi: 10.1007/978-1-4757-5806-1_7

2003

11 K. Ritchie and A. Kusumi. Single particle tracking image microscopy. Methods Enzymol. 360 "Biophotonics" G. Marriott and Ian Parker, editors. pp. 618-634 (2003).  doi: 10.1016/s0076-6879(03)60131-x
10 K. Ritchie, R. Iino, T. Fujiwara, K. Murase, and A. Kusumi. The fence and picket structure of the plasma membrane of live cells as revealed by single molecule techniques. Mol. Memb. Biol. 20, 13-18 (2003). doi: 10.1080/0968768021000055698
9 W. K. Subczynski and A. Kusumi. Dynamics of raft molecules in the cell and artificial membranes: approaches by pulse EPR spin labeling and single molecule optical microscopy. Biochim. Biophys. Acta: Reviews on Biomembranes 1610, 231-243 (2003). doi: 10.1016/s0005-2736(03)00021-x

2001

8 T. Yanagida, E. Tamiya, H. Muramatsu, P. Degenaar, Y. Ishii, Y. Sako, K. Saito, S. Ohta-Iino, S. Ogawa, G. Marriott, A. Kusumi, and H. Tatsumi. Near-field microscopy for biomolecular systems. In "Nano-Optics", S. Kawata, M. Ohtsu, and M. Irie, Eds. Springer, pp. 191-236 (2001). doi: 10.1007/978-3-540-45273-7_8
7 R. Iino and A. Kusumi. Single fluorophore dynamic imaging in living cells. J. Fluorescence 11, 187-195 (2001). doi: 10.1023/A:1012297000742
6 I. Dan, N. M. Watanabe, and A. Kusumi. The Ste20 group kinases as regulators of MAP kinase cascades. Trends Cell Biol. 11, 220-230 (2001). doi: 10.1016/s0962-8924(01)01980-8

1999

5 A. Kusumi and I. Hatta. Foreword for "New Approaches to Membrane Organization. The Proceedings of the Third International Membrane Research Forum". Biophys. Chem. 82, 81-82 (1999).
4 A. Kusumi, K. Suzuki, and K. Koyasako. Mobility and cytoskeletal interactions of cell adhesion molecules. Curr. Opin. Cell Biol. 11, 582-590 (1999). doi: 10.1016/s0955-0674(99)00020-4

1997

3 A. Kusumi, Y. Sako, T. Fujiwara, and M. Tomishige. Application of laser tweezers to studies of fences and tethers of the membrane skeleton which regulate the movements of the plasma membrane proteins. Methods Cell Biol. 55 "Laser Tweezers", M. P. Sheetz, Ed. pp. 173-194 Academic Press (1997). doi: 10.1016/s0091-679x(08)60408-2

1996

2 A. Kusumi and Y. Sako. Cell surface organization by the membrane skeleton. Curr. Opin. Cell Biol. 8, 566-574 (1996). doi: 10.1016/s0955-0674(96)80036-6

1991

1 M. Pasenkiewicz-Gierula, W. K. Subczynski, and A. Kusumi. Influence of phospholipid unsaturation on the cholesterol distribution in the membrane. Biochimie 73, 1311-1316(1991). doi: 10.1016/0300-9084(91)90094-h

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