[Seminar] "Advancing molecular medicine with quantitative single molecule localization microscopy" by Prof. Tijana Jovanovic-Talisman

Title: Advancing molecular medicine with quantitative single molecule localization microscopy

Abstract:

Single molecule localization microscopy (SMLM) can detect single molecules with nanoscale precision. However, it is technically challenging to rigorously quantify receptors in clinical samples: cells from patient tissues and extracellular vesicles secreted by patient cells. To successfully analyze these samples, we tailor our imaging techniques, data analyses, and counting algorithms. We applied our approach to tissue samples from patients with breast cancer; HER2 copy numbers showed a significant positive correlation with detected densities from quantitative SMLM. Additionally, we assessed extracellular vesicles isolated from plasma of patients with pancreatic cancer. We identified a pancreatic cancer-enriched vesicle population and defined vesicle heterogeneity.