[Seminar] "Enzyme Discovery & Engineering to Create Suitable Biocatalysts" by Prof. Uwe Bornscheuer
Seminar Title: Enzyme Discovery & Engineering to Create Suitable Biocatalysts
Speaker: Prof. Uwe T. Bornscheuer, Institute of Biochemistry, Dept. of Biotechnology & Enzyme Catalysis, University of Greifswald, Greifswald, Germany [website]
This lecture will cover recent achievements in the discovery, protein engineering and application of enzymes in biocatalysis . For the asymmetric synthesis of chiral amines, we recently developed a sophisticated growth selection method and could create highly active and selective enzymes from three classes to make important chiral precursors for pharmaceutical building blocks . We have engineered a P450 enzyme for the highly selective formation of ursodeoxycholic acid (UDCA) from lithocholic acid . For the recycling of plastics, we have investigated PET , for which we determined the first structure of an MHETase in complex with a substrate analogue  and also provided important adjustments of a published PETase structure . We also used various methods of protein engineering to improve several PET-hydrolase for higher activity and thermostability . Most recently, we have identified the first urethanases in a metagenomic library able to degrade polyurethanes  and designed an enzyme cascade to degrade poly(vinylalcohols) . Finally, we have explored the degradation of complex algal polysaccharides such as agar/porphyran  and ulvan  using a set of enzymes discovered in marine bacteria.
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