Members

Faculty Member

Dr. Paola Laurino

Unit Members

Dr. Madhuri Gade, Postdoctoral Scholar

Email: madhuri.gade[at]oist.jp

I joined OIST as a Postdoc in November 2017 with Dr. Paola Laurino after completing my Ph.D. in Organic chemistry under the supervision of Dr. Raghavendra Kikkeri from IISER Pune. Before I worked as Project assistant in National Chemical Laboratory, Pune. I completed my masters in 2011 from Pune University in organic chemistry.  My current research interest is focus on cofactor engineering for RNA modifications. My specific aims are engineering S-adenosyl methionine to study epigenetics modifications and engineering of enzymes to accept unnatural cofactor. A bioorthogonal system to study RNA modification in vivo. 

Dr. Bhanu Chouhan, Postdoctoral Scholar

Email: bhanu.chouhan[at]oist.jp

Bhanu (born in Indore, India, 1985) received his Masters degree in Bioinformatics from the University of Turku, Finland in 2009. In 2016, he obtained his PhD degree from the Structural Bioinformatics Laboratory (SBL), Department of Biochemistry, Åbo Akademi University, Finland, under the supervision of Professor Mark S. Johnson and Docent Konstantin Denssiouk. 

His doctoral research has focused on deciphering the evolutionary history of heterodimeric cell-surface receptors known as Integrins  that mediate cell-cell, cell-extracellular matrix and cell-pathogen interactions. Herein, he sought to identify when the constitutive integrin domains first arose and when the mammalian-type functionalities first appeared.

Since March 2018, Bhanu is a Postdoctoral researcher at the Protein Engineering and Evolution Unit under the supervision of Dr. Paola Laurino. His research work at OIST focuses on the ancestral resurrection of ancient enzymes coupled with the wet lab based directed evolution in order to gain novel insights into their (enzyme's) evolutionary trajectories and resurrect mutants with desired properties.   

Dr. Mirco Dindo, JSPS Postdoctoral Fellow

Email: mirco.dindo[at]oist.jp

I was born in Verona (Italy) and I obtained my PhD in Biomolecular Medicine (Curriculum in Biochemistry) at the University of Verona.

I really like proteins from several point of view, catalysis (in particular), conformational changes, different kind of interactions, regulation.. these molecules are amazing! Okinawa is a wonderful place for science and for nature lovers. Currently I am working in the engineering of proteins for the production of non canonical amino acids or for their use as biosensors or marker for early cancer detection. I'm also trying to develop a recipe for scientists, let's see if it works!

Dr. Saacnicteh Toledo Patino, Postdoctoral Scholar

Email: Saacnicteh.ToledoPatino[at]oist.jp

I was born on May 28th, 1984 in Morelia, Mexico where I studied Chemical Pharmacobiology at UMSNH University (2002-2006). I studied Biochemistry at Eberhard-Karls University of Tuebingen (2008-2013), where I obtained my Masters degree in Protein Design (2013). I obtained my PhD in Biochemistry at the Max Planck Institute for Developmental Biology in the same city (2014-2019). During my doctoral studies I joined the University of Bayreuth as a scientific employee (2016-2019). Since December 2019 I work in Prof. Laurino's unit for Protein Design and Evolution where I carry out projects on the emergence of the Rossmann fold and its ligand diversification. 

Dr. Benjamin Clifton, Postdoctoral Scholar

Email: benjamin.clifton@oist.jp

I was born in Sydney, Australia and completed an undergraduate degree in chemistry (PhB (Sci) Hons) and PhD degree in protein biochemistry (2013-2016) at the Australian National University in Canberra. Protein evolution has been a longstanding interest for me, in particular, understanding how complex and functionally diverse proteins evolve through a simple process of mutation and selection, and how we can use this knowledge practically for protein engineering purposes.

During my PhD research in Prof. Colin Jackson's group, I studied the evolution of new protein functions in the solute-binding protein superfamily using ancestral protein reconstruction, a technique that allows extinct proteins to be recreated so that their evolutionary history can be studied in the lab. I then worked in a research role at Affinity Bio (Melbourne, Australia, 2017-2019), where I contributed to the development of antibodies against challenging targets for use in cancer immunotherapy. I joined the Protein Engineering and Evolution Unit at OIST in January 2020 to study how cellular tRNA modifications affect the outcome of protein evolution by controlling the error rate of protein synthesis. I'm also interested in developing new methods for functional annotation of solute-binding proteins for use in biosensor applications.

 

Dr. Gen-ichiro Uechi, Technician

Email: Gen-ichiro.Uechi[at]oist.jp

I'm Uechi. I joined in Apr. 2019. I was born in Okinawa and got a Ph.D. at the University of the Ryukyus.

I want to spread the beauty of Okinawa and want to be a bridge with younger generation.

Mr. Stefano Pascarelli, PhD Student

Email: stefano.pascarelli1[at]oist.jp

I have always been fascinated by is evolution, and how it is able to explain how complex life developed from a simple mixture of atoms. It takes place in so many scales, from proteins to populations. Exploring evolution means understanding the forces involved in developing complexity, and who knows, maybe even being able to reproduce them. I want evolution to be a prominent topic during my time at OIST. Specifically, protein evolution and the different levels of complexity a protein can achieve in evolve.

OIST is a place that really represents my ideologies in science. It is a peaceful place where collaborate with each other towards a common goal is possible. It is the best environment for scientists to make a difference in their field.

We must never forget that science is a community. On one side, it allows the knowledge to be accessible and shared, like a magic wallet that makes everyone that possess it equally richer by anyone’s contribution. On the other side, a community of humans is not new to mistakes, but in this case, the history of the past can help us correct them.

I believe in a borderless scientific community, if not the whole Earth’s community, without discriminations. The instinct of segregating in small, aggressive groups served us well during evolution. Now, it is a dangerous remain of the past, that might annihilate Humanity. I am considerate towards tolerating the differences, learning from another culture, respecting other ideals up to the limit of rationality. The only way to do so is to dive deep in the melting pot. To be an experience to the others as much as they are to you. Static environments more hardly lead to progress.

Ms. Desirae Martinez, Technician

Email: desirae.martinez[at]oist.jp

I was born in Arizona, USA. I received my bachelor’s degree in molecular and cellular biology with a minor in Biochemistry from the University of Arizona, where I worked on studying the allosteric activation of type two restriction enzymes in a biochemistry laboratory. I obtained my master’s degree in clinical biochemistry biomedical science from Middlesex university in London where I worked on heparin sulfate glycosaminoglycans and their involvement in preeclampsia. At OIST, I am excited to use my skills in order to study protein evolution through molecular engineering in many different ways.

Mr. Andrea Testa, JSPS fellow

Email: andrea.testa[at]oist.jp

I was born in northern Italy, and grew up in a little village close to Milan. I obtained my Bachelor's and Master's degrees in Materials Engineering and Nanotechnology from Politecnico di Milano. After working two years in the R&D deparment of an automotive company in nothern Italy, I decided to resign and start a PhD at ETH Zürich in the Laboratory of Soft and Living Materials. I joined the Protein Engineerin and Evolution group in OIST in February 2020 for a three months research stay working on phase separation of protein droplets.

I fell in love with Japan after spending six months in Tokyo for my master thesis work in 2015, and I am very happy to be back in this amazing country. I am also thrilled to work in OIST, an amazing place!

 

Mr. Keon Young Kim, Rotation Student

Email: keon.kim[at]oist.jp

I was born on Oct 26th, 1991, and grew in Chuncheon, South Korea. I studied Bioinformatics and Life Science at Soongsil University in Seoul (2010-2014) and got a master's degree in Protein X-ray Crystallography at the same university (2014-2016). Right after graduation, I worked for three years in research institutes to fulfill my national duty. I entered the OIST as a Ph.D. candidate since the last September, and now I'm having a valuable time participating in the laboratory rotation.

 

Mr. Yoshiki Ochiai, Rotation Student

Email: Yoshiki.ochiai[at]oist.jp

I was born and grew up in Osaka, Japan. I studied in molecular biology, biochemistry, and biophysics at Soka University, Tokyo. I got my master degree in Saitama university, and, at the same time, I worked in RIKEN Center for Sustainable Resource Center (CSRS) and The University of Tokyo.

I joined in Protein Engineering and Evolution Unit from May 2020. Here I have engaged in designing novel enzymes for solving biological problems.

 

Ms. Anna Magdalena Klarkowska, Research Intern

Email: AnnaMagdalena.Klarkowska[at]oist.jp

I was born in Warsaw, Poland, where I started a degree in Biotechnology at the Warsaw University of Life Sciences. I transferred to the University of Aberdeen and obtained a BSc in Genetics in 2019. Shortly afterwards, I came to Okinawa as an intern. I am currently working on engineering protein biosensors for detection of cancer-specific glycans.

Ms. Sachie Matsuoka, Unit Research Administrator

Email: sachie.matsuoka[at]oist.jp