Electron Microscopy



Imaging Section (IMG-request*oist.jp) (replace* by @)

 


Imaging Section (IMG-request*oist.jp) (replace* by @)

 

Image Corrected Environmental Transmission Electron Microscope

Manufacturer: Thermo Fisher Scientific

Model: Titan ETEM

Features:

Acc. Voltage: 80 - 300 keV

Holder: Gatan Heating Holder Single Tilt/Double Tilt, Gatan Cooling Holder, Fischione Tomography Holder, Protochips Aduro (Heating and Biasing Holder), Protochips Poseidon (Liquid Cell Holder with heating, biasing, and flow).

Camera: Ceta IS, Quantum ER Energy Filter.

Software: TEM/STEM Tomography Software, SerialEM, Holoworks.

Other equipment: STEM (BF/DF/HAADF detector), Oxford X-Max EDX detector, Biprism.

A device capable of observing atoms with high resolution,  TEM mode (with Cs correction function) and STEM mode, an EDX detector and an EELS detector. The ETEM allows observation of samples in gaseous environments with a range of in-situ holders.

Atomic Resolution Analytical Transmission Electron Microscope
Manufacturer: JEOL
Model: JEM-ARM200F

Features:

Acc. Voltage: 60 - 200 keV

A device capable of observing atoms with high resolution,  TEM mode and STEM mode (with Cs correction function), an EDX detector and an EELS detector. (Scanning resolution: 0.078 nm)

Transmission Electron Microscope (TEM)
Manufacturer: JEOL
Model: JEOL1400 Flash

Features:

Acc. Voltage: 100 keV

An electron microscope that uses a beam of electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects.
 

Software: Limitless Panorama, for automated montaging of images.

Cryo-Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Titan Krios

Features:

Acc. Voltage: 300 keV

Holder:
Cryo-stage/Autoloader plan 3

Camera:
FEI Falcon 3EC, FEI Ceta camera, Gatan energy filter with Gatan K2

Software:
EPU automated data acquisition software, TEM/STEM Tomography software,  SerialEM, Leginon​

Other equipments:
Phase plate, STEM (BF/DF/HAADF detector)

Cryo-Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Talos Arctica

Features:

Acc. Voltage: 200 keV

Holder:
Cryo-stage/Autoloader plan 3

Camera:
FEI Falcon 3EC, FEI Ceta camera

Software:
EPU automated data acquisition software, TEM/STEM Tomography software,  SerialEM, Leginon

Cryo-Transmission Electron Microscope
Manufacturer: Thermo Fisher Scientific
Model: Talos L120C

Features:

Acc. Voltage:120 keV

Holder:
Single-tilt holder, high-field-of-view tomography holder, cryo-holder (Fischione, Model 2550)

Camera:
FEI Ceta camera

Software:
EPU automated data acquisition software, TEM Tomography software, SerialEM

 

 

Serial Block-Face Scanning Electron Microscope (SBEM)
Manufacturer: Thermo Fisher Scientific
Model: Teneo-VS

Features:

The Teneo has a build-in microtome that allows the removal of a thin layer of resin with subsequent imaging of the remaining block-face to acquire high-resolution data of large volumes. Generally the volumes are larger and the resolution is lower that with a FIB-SEM.
This SEM can also be used for array tomography.

Scanning Electron Microscope (SEM)
Manufacturer: JEOL
Model: JSM-7900F

Features:

A device is capable of SEM imaging in high vacuum and low vaccume mode with mutiple detectors  from low accelerating voltage (100 V) 

The device is also  equippped with 2 set of EDX detectors for elemental analysis and EBSD for crystal phase analysis. 

Focused Ion Beam / Scanning Electron Microscope (FIB-SEM)
Manufacturer: Thermo Fisher Scientific
Model: Helios NanoLab 650

Features:

The Helios is equipped with a dual beam system: a focused gallium ion beam (FIB) for sample processing and an electron beam (SEM) for simultaneous imaging.
Our system is mainly used for FIB-SEM tomography of resin embedded biological samples.
A cryo-stage from Quorum Company, UK, allows to process and image cryo-samples.