OIST-UT Joint talk series for future science-Season 3: Intraoperative Rapid Imaging of Tiny Tumors by Novel Fluorogenic Probes for Exopeptidases and Glycosidases

Title:

Intraoperative Rapid Imaging of Tiny Tumors by Novel Fluorogenic Probes for Exopeptidases and Glycosidases

Abstract:

Suitable activatable fluorescence probes are naturally of critical importance for fluorescence imaging, and we have succeeded to develop novel fluorogenic probes for various exopeptidases and glycosidases based on our newly established rational design strategy with intramolecular spirocyclization and photoinduced electron transfer mechanism. For example, gGlu-HMRG, a novel sensitive fluorogenic probe for g-glutamyltranspeptidase (GGT), which is well-known to be upregulated in various cancer cells, was developed. By topical application of gGlu-HMRG to freshly excised human breast specimens, tumorous lesions selectively exhibited a time-dependent increase of green fluorescence originated from the fluorescent cleavage product of gGlu-HMRG, and were clearly distinguished from surrounding mammary gland and fat within a few minutes. Very recently, we also succeeded to develop a library of fluorogenic probes for glycosidases, and to find out HMRef-aMan, an a-mannosidase-reactive fluorogenic probe, as a suitable probe for detecting breast tumors by imaging-based screening with real human clinical samples. Furthermore, combined application of green-emitting HMRef-aMan and a red-emitting GGT probe enabled efficient dual-color, dual-target optical discrimination of malignant and benign breast tumors. We believe these rapidly activatable probes would enable more confident and minimally invasive resection of tumors, which would be beneficial for both patients and clinicians.

Speaker: 

Dr. Yasuteru Urano, The University of Tokyo

Profile:

Yasuteru Urano is a Professor at Graduate School of Pharmaceutical Sciences and Graduate School of Medicine, the University of Tokyo (UT), JAPAN. Dr. Urano obtained BE, ME, and PhD degree (Pharmaceutical Sciences) in 1990, 1992, and 1995 from the UT, respectively. After a JSPS post-doctoral fellow from 1995 to 1997, he became an assistant professor in 1997, an associate professor from 2005 at Graduate School of Pharmaceutical Sciences, UT, and then a full professor at Graduate School of Medicine, UT from 2010. From 2013, he also became a professor at Graduate School of Pharmaceutical Sciences, UT.>

Chair:

Prof. Akihiro Kusumi, OIST

Zoom:

• https://oist.zoom.us/j/98591991386?pwd=cUZseDlLUElGWmZYTjE2VnZOZ3JDdz09
• Meeting ID: 985 9199 1386
• Passcode: 596372