[Seminar] Xingya Xu, How do condensin and cohesin work: ‘hold-and-release(clip)’ or ‘ring’?
Date
Friday, November 1, 2019 - 16:30 to 17:00
Location
Lab3 C700
Description
Speaker: Xingya Xu (G0 Cell Unit)
Title: How do condensin and cohesin work: ‘hold-and-release(clip)’ or ‘ring’?
Abstract:
Cohesin and condensin play fundamental roles in sister chromatid cohesion and chromosome segregation, respectively. Both consist of heterodimeric structural maintenance of chromosomes (SMC) subunits, which possess a head and a hinge, intervened by long coiled coils. Non-SMC subunits bind to the SMC heads. Separase cleaves cohesin at anaphase to release chromatin. Here, we report a large number of spontaneous extragenic suppressors for fission yeast separase, cohesin and condensin mutants. Separase ts mutants were rescued by interfaces mutations at cohesin head or hinge. Both cohesin and condensin hinge ts or cs mutants were rescued by mutations in helix-turn-helix (HTH) motif of kleisin that directly bind to head domain. We proposed a hold-and-release model for both condensin and cohesin, in which head (or kleisin) and hinge may interact directly and collaboratively regulate the resulting coiled coils to hold and release chromosomal DNAs. HTH motifs of kleisin may have a critical role in mediating these interactions.
After the talk, join for discussion with free soft drinks and pizza!
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