Program

March 1st, 2017

8:30 Registration

9:00 - 9:05 Message from the Organisers

9:05 - 9:15 Message from OIST Dean of Research

Session Chair: Kelly Hughes

9:15 - 9:40 Howard Berg - Bacterial Motility: Swimming and Gliding

9:40  - 10:05 Richard Berry - Torque, speed and assembly of the E. coli flagella motor

10:05 - 10:30 Yoshiyuki Sowa - Single-molecule studies of the chemo-mechanical coupling in the bacterial flagellar motor

10:30 - 10:50: Coffee-Tea break

Session Chair: Judith Armitage

10:50 - 11:15 Rasika Harshey - Success in a swarm

11:15 - 11:40 Akihiko Ishijima - The chemotactic response and motor function of a bacterial flagella motor in a single cell

11:40 - 11:55 Akio Kitao - Dynamic structure of bacterial flagellar proteins observed in silico

11:55 - 12:20 Seishi Kudo - A Trial Run of a Bacterium Robot with a Flagellum

12:20 - 12:25 Discussion / Announcement

12:25 - 13:55: Lunch – Break

Session Chair: Seiji Kojima

13:55 - 15:00 Poster talks

15:00 - 16:30 Poster presentation / Coffee-Tea break

Session Chair: Shin-Ichi Aizawa

16:30 - 16:55 David Blair - Dissecting the mechanism of flagellar type-III secretion

16:55 - 17:20 Katsumi Imada - In vitro observation of the bacterial flagellar type III protein export

17:20 - 17:35 Danielle Tullman-Ercek - Engineering high-titer heterologous protein secretion in bacteria

17:35 - 17:40 Discussion / Announcement / Closing

-Dinner-

March 2nd, 2017

Session Chair: Ariel Blocker

9:00 - 9:25 Samuel Wagner - Assembly, structure, and function of the export apparatus of the Salmonella SPI-1 T3SSs

9:25 - 9:50 Linda Kenney - Super-resolution Imaging of Salmonella SPI-2 regulation: A View from 30,000 feet to 20 nm

9:50 - 10:15 Natalie Strynadka - Near-atomic CryoEM Structural Analysis of the Salmonella Type III Injectisome Basal Body

10:15 - 10:30 Neta Sal-Man - Calcium Sensing and Secretion Hierarchy Regulation in EPEC T3SS

10:30 - 10:50: Coffee-Tea break

Session Chair: Keiichi Namba

10:50 - 11-15 Marc Erhardt - Self-Assembly of a Bacterial Nanomachine: Flagella Grow Through an Injection-Diffusion Mechanism

11:15 - 11:30 Chien-Jung Lo - The growth rate of Vibrio alginolyticus polar flagella

11:30 - 11:55 Tohru Minamino - Cooperative remodeling of the FlhA ring structure coordinates flagella type III protein export with assembly

11:55 - 12:00 Discussion / Wrapping up

12:00 - 13:25 Lunch – Break

Session Chair: Tohru Minamino

13:25 - 13:40 Jun Liu - In Situ Structural Analysis of Vibrio Flagellar Motor by Cryo-Electron Tomography and Sub-tomogram Averaging

13:40 - 13:55 Daisuke Nakane - Asymmetric Distribution of Type IV Pili Triggered by Directional Light in Unicellular Cyanobacteria

Session Chair: Marc Erhardt

13:55 - 15:00 Poster talks

15:00 – 16:30 Poster presentation / Coffee-Tea break

Session Chair: Ikuro Kawaghisi

16:30 - 16:55 Sonja Albers - Assembly and Interactions of the Archaellum Motor

16:55 - 17:10 Tomohiro Kimura - AMIS75470 is Essential for Flagellar Assembly in the Rare Actinomycete Actinoplanes missouriensis

17:10 - 17:35 Ariane Briegel - Structure and Function of Chemoreceptor Arrays- the Bacterial Brain that Controls Flagellar Motility

17:35 - 17:40 Discussion / Announcement

-Conference dinner (special dinner)-

March 3rd, 2017

Session Chair: Michio Homma

8:45 - 9:10 Judith Armitage - Exchange of motor proteins measured in living cells

9:10 - 9:35 Keiichi Namba - Insights into the mechanism of bacterial flagella motor rotation from the structure and dynamics

9:35 - 10:00 Seiji Kojima - How to anchor and activate flagellar stator units: assembly-coupled stator activation mechanism revealed by the crystal and solution structures of the stator fragment

10:00 - 10:25 Takayuki Nishizaka - Unitary steps of supermolecular motility machineries in gliding bacteria and swimming archaea

10:25 - 10:40: Coffee-Tea break

Session Chair: Linda Kenney

10:40 - 11:05 Ariel Blocker - Structural and Functional Analysis of Type III Secretion Systems

11:05 - 11:30 Tomoko Yamamoto - Functional dissection of Salmonella SPI-2 injectisome

11:30 - 11:45 Hanh Lam - Synthetic cyclic peptomers as Type III secretion system inhibitors

11:45 - 12:00 Nicholas Dickenson - Spa47 oligomerization drives ATP hydrolysis and Shigella T3SS activation

12:00 - 12:15 Tomoko Kubori - Manipulation of the host Ubiquitin system by legionella Deubiquitinases

12:15- Excursion to Okinawa aquarium (Lunch in Bus)

          Free afternoon (for those not going to the aquarium)

18:30 Dinner

March 4th, 2017

Session Chair: Seishi Kudo

9:00 - 9:25 Kelly Hughes - Nano-scale length control of the flagellar drive-shaft requires hitting the protein-tethered outer membrane

9:25 - 9:50 Daniel Kearns - Homeostatic control of the flagellar filament protein in Bacillus subtilis

9:50 - 10:15 Michio Homma - How to determine the number and localization of polar flagellum in Vibrio cells?

10:15 - 10:30 Bin Ni - Evolutionary Remodeling of Bacterial Motility Checkpoint Control

10:30 - 10:50 Coffee-Tea break

Session Chair: Rasika Harshey

10:50 - 11:15 James Fleckenstein - A Secreted Adhesin Bridges Enterotoxigenic E. coli Flagella and A-blood group Host Glycans

11:15 - 11:40 Fadel A. Samatey - Bacterial flagellar hook: similarities and specificities

11:40 - 11:55 Thibaud Renault - A highly conserved methionine loop in FliP controls substrate export across the inner membrane

11:55 - 12:10 Clive Barker - Stability of the bacterial flagellar hook as a universal joint

12:10 - 12:15 Discussion / Announcement / Closing

12:15 - 13:45 Lunch - Break

Session Chair: Ariane Briegel

13:45 - 14:50 Poster talks

14:50 – 16:20 Poster presentation / Coffee-Tea break

Session Chair: Daniel Kearns

16:20 - 16:45 Shin-Ichi Aizawa - Campylobacter jejuni flagella is controlled short for optimal pathogenesis

16:45 - 17:10 Ikuro Kawagishi - Dynamically Regulated Assembly of the Bacterial Xenobiotic Efflux Complex

17:10 - 17:25 Satoshi Shibata - A multi-rail structure in the cell envelope for the bacteroidete gliding machinery

17:25 - 17:35 Conference Closing / Announcement / Dinner

= = = March 5th, 2017:  Departure

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