Samples preparation equipment

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Automated Plunge Freezers
Manufacturers: ThermoFisher and Leica
Model: Vitrobot MarkIV and EM GP2

Features:

A plunge freezing tool to prepare cryo TEM sample grids which blots the liquid from the grid and plunges into it liquid ethane to obtain grids with controlled ice thickness for Cryo TEM.

Ultra-microtome
Manufacturer: Leica
Model: UC7

Features:

A microtome is a tool used to cut extremely thin slices of material, known as sections

Cryo ultra-microtome
Manufacturer: Leica
Model: EM FC7

Features:

A microtome is a tool used to cut extremely thin slices of material, known as sections (Cryo sections)

light microscope
Manufacturer: Olympus
Model: BX51

Features:

The optical microscope, often referred to as light microscope, is a type of microscope which uses visible light and a system of lenses to magnify images of small samples (×100-×1000)

Stereo microscope
Manufacturer: Olympus
Model: SZ61

Features:

The stereo microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an object rather than transmitted through it.

Centrifuge
Manufacturer: TOMY
Model: Kintaro

Features:

A centrifuge is a piece of equipment that puts an object in rotation around a fixed axis (spins it in a circle), applying a potentially strong force perpendicular to the axis of spin (outward). The centrifuge works using the sedimentation principle, where the centripetal acceleration causes denser substances and particles to move outward in the radial direction. (Maximum speed is 13500rpm)

Centrifuge
Manufacturerr: TOMY
Model: Kintaro

Features:

Multifunction vacuum deposition equipment
Manufacturer:
Model: VE3030CVD

Features:

Vaporation is a common method of thin-film deposition. A source material would be evaporated in a vacuum. The vacuum allows vapor particles to travel directly to the target object (substrate), where they condense back to a solid state. (Uses Au, Carbon, Pt and many metal targets target in 0.001Pa)

High pressure freezer Manufacturer: Leica Model: HPM100

Features:

High pressure freezer is used for rapid freezing of samples under high pressure as a method of cryo-fixation. In this method we can omit the chemical fixation often used for sample preparation for electron microscopy. The advantage is a closer to native state of the sample. Samples fixed with this method are usually processed later on by freeze substitution.

Vacuum Evaporator
Manufacturer: JEOL
Model: IB-29510VET

Features:

Evaporation is a common method of thin-film deposition. The source material is evaporated in a vacuum. The vacuum allows vapor particles to travel directly to the target object (substrate), where they condense back to a solid state. (Uses Au and Carbon target in 0.00001Pa)

Freeze Dryer
Manufacturer: HITACHI
Model: ES-2030

Features:

The water contained in the hydrated sample for SEM would be dehydrated with ethyl alcohol, and substituted with t-butyl alcohol, and froze in a refrigerator of -20℃ and then dried with sublimation at about 13.3 Pa in the evacuator of reduced pressure.

Critical point dryer
Manufacturer: JEOL
Model: JCPD-5

Features:

An apparatus for drying a scanning electron microscope sample by utilizing a critical state of a liquid which can be formed by an appropriate combination of temperature and pressure.

Freeze Substitution and Low Temperature Embedding System for Light and Electron Microscopy
Manufacturer:Leica
Model: EM AFS2

Features:

This device performs freeze substitution and progressive lowering of temperature techniques and allows low temperature embedding and polymerization of resins.

Slush Nitrogen production equipment
Manufacturer: JEOL
Model: slushy Nitrogen Production

Features:

Slush hydrogen is a combination of liquid hydrogen and solid hydrogen at the triple point with a lower temperature and a higher density than liquid hydrogen for making a rapid freezing biology samples.

Precision ion polishing system
Manufacturer: Gatan
Model: PIPSII

Features:

Precision ion polishing system using dual argon plasma beams to polish thin film and lamella samples, with a collimated beam from 100V.

Disk Polishing Machine
Manufacturer: Marumoto Struers
Model: LaboSystem

Features:

Grinding/polishing machine with variable speed.

Hydrophilisation device
Manufacturer: JEOL
Model: HD Treatment

Features:

Hydrophilization of the surface of the TEM support film. Dispersion of powder samples in suspensions and negative staining such as virus, fiber, liposome etc. also show excellent effects on dispersibility and dyeability of samples.

InvitroShaker
Manufacturer:
Model: Shake-XR

Features:

It is possible to perform a three-dimensional vibration like a mix of swirling and seesaw, which is best suited for a gentle shaking of the centrifuge tube horizontally.

Automated vibratome for fresh tissue sectioning
Manufacturer:Leica
Model: VT 1000S

Features:

A breakthrough system in which a vibrating blade cuts a specimen while electric knife feeding is performed

Plasma Cleaner
Manufacturer: Gatan
Model: Solarius II

Features:

Plasma cleaning is the removal of impurities and contaminants from surfaces through the use of an energetic plasma or dielectric barrier discharge (DBD) plasma created from gaseous species. The Solarius II is equiped with Argon, Oxygen and Hydrogen gas.

Electron Microscope Oven Processor
Manufacturerr:
Model: TD-700

Features:

Polymerizes resin at a temperature of 40-60 ℃.

Ultrasonic Cutter
Manufacturer: Gatan
Model: 601

Features:

Quickly cut simple holes discs the size of TEM grids from hard, brittle materials for additional thininning with the Dimple Grinder, PIPSII and/or Disk Polishing Machine.

Dimple Grinder
Manufacturer: Gatan
Model:

Features:

A mechanical method of pre-thinning a section of the sample to greatly reduce time needed for ion milling with the PIPSII.

Low Speed Saw
Manufacturer: Buehler
Model: IsoMet

Features:

Compact gravity fed precision cutter. Adjustable motor up to 300 rpm.