2012年のセッション

顕微鏡の基本

• 日時：2012年5月15日－2012年5月17日 
• 講師：石舘 文善
• 講義名：顕微鏡の基本
• 顕微鏡：２光子励起共焦点蛍光顕微鏡(LSM710およびLSM510 META NLO, Zeiss)
• 略歴：1970年から2012年までカールツァイスマイクロスコピー(株)。現在、京都大学客員教授、東京大学客員研究員、理化学研究所客員研究員。「顕微鏡の使い方ノート」の著者の一人であり、世界で最高水準の技術を持つ、共焦点顕微鏡技術者の一人でもある。

生命科学における１分子可視化計測

• 日時：2012年6月5日－2012年6月7日
• 講師：佐甲 靖志
• 講義名：生命科学における１分子可視化計測
• 顕微鏡：全反射蛍光顕微鏡(TIRF-C1Si, Nikon)
• 略歴：1991年京都大学理学博士。東京大学、名古屋大学、大阪大学を経て、2006年より理化学研究所主任研究員。細胞内情報処理ネットワー ク、蛋白質の構造と機能、光学顕微鏡技術の開発などに従事。

生きた細胞観察のための高速な周波数領域蛍光寿命測定法

• 日時：2012年10月23日－10月24日
• 講師：Michiel Kregel, Ph.D., (Lambert Instruments BV, The Netherlands)
• 講義名：生きた細胞観察のための高速な周波数領域蛍光寿命測定法
• 顕微鏡：LIFA (Lambert Instruments Fluorescence Attachment) on Olympus IX71
• 略歴：Dr. Michiel Kregel has a PhD in Astrophysics from the University of Groningen on visible and near-infrared spectrometry of spiral galaxies, and has been active for 4 years as a consultant in spatial information technology before taking a position as product manager at Lambert Instruments BV two years ago. At Lambert Instruments he is responsible for product management and world-wide sales of the LIFA product line for FLIM-fluorescence lifetime imaging microscopy, and is actively involved in various collaborations with universities and industry.
• アブストラクト：Mapping the fluorescence lifetime allows quantitative study of molecular kinetics in living cells and fixed materials on the micro- and nano-scales. The fluorescence lifetime is highly quantitative because it is independent of variations in light intensity, concentration, and photo-bleaching.
      The applications of lifetime imaging are various; from ion imaging and oxygen imaging to drug discovery and medical diagnosis. The premier application of FLIM is the study of molecular interactions through FRET-Förster Resonance Energy Transfer. FLIM-FRET is nowadays used extensively in studying protein-protein interactions throughout cell-biology and pharmacy.
      In this workshop, participants will learn about the principles of FLIM and FRET and are introduced to the LIFA system for fast FLIM, focussing on key analysis features such as mapping the FRET efficiency. The LIFA is a fast camera-based FLIM system operating in the frequency domain that is very easy to setup and use. The LIFA can be attached to all brands of fluorescence microscopes and is also compatible with optical sectioning techniques such as TIRF and multi-beam confocal, and with automated microscopy.

First step for fluorescence correlation spectroscopy and fluorescence cross correlation spectroscopy of living cell

• 日時：2012年11月13日－2012年11月15日
• 講師：Masataka Kinjo/ Shintaro Mikkuni /Fumiyoshi Ishidate
• 講義名：First step for fluorescence correlation spectroscopy and fluorescence cross correlation spectroscopy of living cell.
• 顕微鏡：Fluorescence Correlation Spectroscopy (ConfoCor3, Zeiss)
• 略歴：Masataka Kinjo obtained his PhD in 1985 from Jichi Medical School, Japan. After worked at Research Institute of Applied Electricity, Hokkaido University as assistant and associate professor, he became professor at Faculty of Advanced Life Science, Hokkaido University.  He spent two years in Karolinska Institute as a guest researcher.