"Patterning surfaces by microcontact printing (μCP): as simple as an “inkan” -- Shivani Sathish

Internal Seminar: Shivani Sathish, Student, Micro/Bio/Nano fluidics Unit (Shen Unit)

Title: "Patterning surfaces by microcontact printing (μCP): as simple as an “inkan”

Abstract: Since recent years, there is a growing need for cheaper, simpler and more reliable means for simultaneous detection of multiple biomarkers (e.g., DNA and protein fragments). To satisfy these demands, significant efforts to fabricate biomolecular micro- and nanoarray-based microfluidic devices are underway. However, there have been several obstacles that hinder their routine usage in clinical diagnostics. Primarily, it has been a major challenge to integrate these micro/nanopatterned surfaces within microfluidic devices without significantly affecting the functionalities of the immobilized biomolecules. Additionally, as the flow within the microfluidic channels generates high shear stresses, the immobilized biomolecules gradually desorb from the surfaces with time, leading to erroneous screening results. To address these concerns, we introduced a simple aqueous based microcontact printing (μCP) method to pattern micro and nanoarrays of (3-aminopropyl) triethoxysilane (APTES) on glass substrates. These substrates were then integrated with microfluidic channels, to covalently couple functional DNA aptamers and antibodies with the micro- and nano patterned APTES, capable of reliably detecting interleukin-6 (IL6) and human C-reactive protein (hCRP) within the devices. In summary, with a single printing step, this patterning technique enables the creation of functional and robust microfluidic micro- and nanobiomolecular arrays, laying the foundation for high-throughput multiplexed bioassays.
 

Refreshments will be served afterwards. We hope to see you there!
 

Sincerely,
 

Internal Seminar Organizing Committee:

Marylka Yoe Uusisaari
Bianca Sieveritz
Rob Campbell
Margaret Mars-Brisbin
Lauren Dembeck
Maeva Techer
Jigyasa Arora
Yuka Suzuki