Publications
Other publications by lab members (with the OIST affiliation, but without Filip)
Preprints and articles in review
Jinyeong Choi, Pradeep Palanichamy, Hirotaka Tanaka, Matthew E. Gruwell, Filip Husnik. Accelerated pseudogenization in ancient endosymbionts of giant scale insects. bioRxiv doi: https://doi.org/10.1101/2024.10.23.619753.
Jinyeong Choi, Pradeep Palanichamy, Seunghwan Lee, Filip Husnik. The diversification of mealybugs was triggered by new symbiont acquisitions and followed by adaptive radiations on host plants. bioRxiv doi: https://doi.org/10.1101/2024.10.23.619971.
Maria E. A. Santos, James D. Reimer, Bogdan Kiriukhin, Hin Boo Wee, Masaru Mizuyama, Hiroki Kise, Marcelo V. Kitahara, Akira Iguchi, 'Ale'alani Dudoit, Robert J. Toonen, Filip Husnik. Coral microbiomes from the Atlantic and Indo-Pacific oceans have the same alpha diversity but different composition. bioRxiv doi: https://doi.org/10.1101/2024.08.16.608269.
Publications
2024
Cho A, Lax G, Livingston SJ, Masukagami Y, Naumova M, Millar O, Husnik F, Keeling PJ. Genomic analyses of Symbiomonas scintillans show no evidence of endosymbiotic bacteria but reveal the presence of giant viruses. PLoS Genetics 2024, 20(4): e1011218.
2023
Phua YH, Tejeda JA, Roy MC, Husnik F, Wakeman KC. Bacterial communities and toxin profiles of Ostreopsis (Dinophyceae) from the Pacific Island of Okinawa, Japan. European Journal of Protistology 2023, 89: 125976.
Park E, Cooney E, Phua YH, Horiguchi T, Husnik F, Keeling P, Wakeman K, Leander B. Phylogenomics shows that novel tapeworm-like traits of haplozoan parasites evolved from within the Peridiniales (Dinoflagellata). Molecular Phylogenetics and Evolution 2023, 18: 107859.
George EE, Barcyte D, Lax G, Livingston S, Tashyreva D, Husnik F, Lukes J, Elias M, Keeling PJ. A single cryptomonad cell harbours a complex community of organelles, bacteria, a phage and selfish elements, Current Biology 2023, 33(10): P1982-1996.E4.
Husnik F. Organellogenesis: Host proteins control symbiont cell divisions, Current Biology 2023, 33(1): PR22-PR25.
2022
Syberg-Olsen MJ, Graber AI, Keeling PJ, McCutcheon JP, Husnik F: Pseudofinder: detection of pseudogenes in prokaryotic genomes [https://github.com/filip-husnik/pseudofinder], Molecular Biology and Evolution 2022, 39(7): msac153.
Giannotti D, Boscaro V, Husnik F, Vannini C, Keeling P. At the threshold of symbiosis: the genome of obligately-endosymbiotic “Candidatus Nebulobacter yamunensis” is almost indistinguishable from that of a cultivable strain, Microbial Genomics 2022, 8(12): 000909.
Boscaro B, Syberg-Olsen MJ, Irwin NAT, George E, Vannini C, Husnik F, Keeling PJ. All essential endosymbionts of the ciliate Euplotes are cyclically replaced, Current Biology 2022, https://doi.org/10.1016/j.cub.2022.06.052.
Tanaka H, Sasaki D, Choi J, Husnik F, Kamitani S. Two new species of mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) from Japan. Zootaxa 2022, 5168(3): 306-318.
George EE, Tashyreva D, Kwong WK, Okamoto N, Horak A, Husnik F, Lukes J, Keeling PJ. Gene transfer agents in bacterial endosymbionts of microbial eukaryotes, Genome Biology and Evolution 2022, https://doi.org/10.1093/gbe/evac099.
Giannotti D, Boscaro V, Husnik F, Vannini C, Keeling PJ: The ‘other’ Rickettsiales: an overview of the family ‘Candidatus Midichloriaceae’, Applied and Environmental Microbiology 2022, https://doi.org/10.1128/aem.02432-21.
Phua YH, Husnik F, Lemer S, Wakeman KC: Phylogeny of benthic dinoflagellate Amphidinum (Dinophyceae) from the South Pacific Islands of Guam and Okinawa, with the description of A. pagoensis sp. nov. and A. uduigamensis sp. nov., Phycologia 2022, https://doi.org/10.1080/00318884.2022.2033948.
2021
Husnik F, Tashyreva D, Boscaro V, George EE, Lukes J, Keeling PJ: Bacterial and archaeal symbioses with protists. Current Biology 2021, 31(13): PR862-R877.
Phua YH, Roy MC, Lemer S, Husnik F, Wakeman KC: Diversity and toxicity of Pacific strains of the benthic dinoflagellate Coolia (Dinophyceae), with a look at the Coolia canariensis species complex. Harmful Algae 2021, https://doi.org/10.1016/j.hal.2021.102120.
Bain SA, Marshall H, de la Filia AG, Laetsch D, Husnik F, Ross L: Sex‐specific expression and DNA methylation in a species with extreme sexual dimorphism and paternal genome elimination. Molecular Ecology 2021, https://doi.org/10.1111/mec.15842.
Mathur V, Kwong WK, Husnik F, Irwin NAT, Kristmundsson A, Gestal C, Freeman M, Keeling PJ: Phylogenomics identifies a new major subgroup of apicomplexans, Marosporida class. nov., with extreme apicoplast genome reduction. Genome Biology and Evolution 2021, 13 (2): evaa244.
2020
Husnik F, Hypsa V, Darby A: Insect—symbiont gene expression in the midgut bacteriocytes of a blood-sucking parasite. Genome Biology and Evolution 2020,12 (4): 429-442.
Before joining OIST
(22) Massana R, Labarre A, López-Escardó D, Obiol A, Bucchini F, Hackl T, Fischer MG, Vandepoele K, Tikhonenkov DV, Husnik F, Keeling PJ: Gene expression during bacterivorous growth of a widespread marine heterotrophic flagellate. ISME J 2021, 15: 154-167.
(21) George EE, Husnik F, Tashyreva D, Prokopchuk G, Horak A, Kwong WK, Lukes J, Keeling PJ: Highly reduced genomes of protist endosymbionts show evolutionary convergence. Current Biology 2020, 30 (5): 925-933.
(20) Gawryluk RMR, Tikhonenkov DV, Hehenberger E, Husnik F, Mylnikov AP, Keeling PJ. Non-photosynthetic predators are sister to red algae. Nature 2019, 572: 240–243 [ReadCube Access].
(19) Boscaro V, Husnik F, Vannini C, Keeling PJ. Symbionts of the ciliate Euplotes: diversity, patterns and potential as models for bacteria–eukaryote endosymbioses. Proc. R. Soc. B 2019, 286: 20190693.
(18) Deeg CM, Zimmer MM, George EE, Husnik F, Keeling PJ, Suttle CA. Chromulinavorax destructans, a pathogen of microzooplankton that provides a window into the enigmatic candidate phylum Dependentiae. PLoS Pathogens 2019, 15(5):e1007801.
(17) Van Steenkiste NWL, Stephenson I, Herranz M, Husnik F, Keeling PJ, Leander BS. A new case of kleptoplasty in animals: Marine flatworms steal functional plastids from diatoms. Science Advances 2019, 5(7): eaaw4337.
(16) Husnik F & Keeling PJ. The fate of obligate endosymbionts: reduction, integration, or extinction. Current Opinion in Genetics & Development 2019, 58-59: 1-8.
(15) Treitli S, Kolisko M, Husnik F, Keeling PJ, Hampl V. Revealing the metabolic capacity of Streblomastix strix and its bacterial symbionts using single-cell metagenomics. Proceedings of the National Academy of Sciences of the United States of America 2019, 116 (39): 19675-19684.
(14) Gile GH, James ER, Tai V, Harper JT, Merrell TL, Boscaro V, Husník F, Scheffrahn RH, Keeling PJ: New Species of Spirotrichonympha from Reticulitermes and the Relationships Among Genera in Spirotrichonymphea (Parabasalia). Journal of Eukaryotic Microbiology 2018, 65(2): 159-169.
(13) Husnik F & McCutcheon JP: Functional horizontal gene transfer from bacteria to eukaryotes. Nature Reviews Microbiology 2018, 16 (2): 67-79 [ReadCube Access].
(12) Husnik F: Host-symbiont-pathogen interactions in blood-feeding parasites: nutrition, immune cross-talk and gene exchange. Parasitology 2018, 145(10): 1294-1303.
(11) Lukes J & Husnik F: Microsporidia: A Single Horizontal Gene Transfer Drives a Great Leap Forward. Current Biology 2018, 28(12): R712–R715.
(10) Říhová J, Nováková E, Husnik F, Hypša V: Legionella becoming a mutualist: adaptive processes shaping the genome of symbiont in the louse Polyplax serrata. Genome Biology and Evolution 2017, 9(11): 2946–2957.
(9) Šochová E, Husnik F, Nováková E, Halaijan A, Hypša V: Arsenophonus and Sodalis replacements shape evolution of symbiosis in louse flies. PeerJ 2017, 5:e4099.
(8) Kyselková M, Chrudimský T, Husník F, Chroňáková A, Heuer H, Smalla K, Elhottová D: Characterization of tet (Y)-carrying LowGC plasmids exogenously captured from cow manure at a conventional dairy farm. FEMS Microbiology Ecology 2016, 92(6): fiw075.
(7) Husnik F, McCutcheon JP: Repeated replacement of an intrabacterial symbiont in the tripartite nested mealybug symbiosis. Proceedings of the National Academy of Sciences of the United States of America 2016, 113(3): E5416-5424.
(6) Nováková E, Hypša V, Nguyen P, Husník F, Darby AC: Genome sequence of Candidatus Arsenophonus lipopteni, the exclusive symbiont of a blood sucking fly Lipoptena cervi (Diptera: Hippoboscidae). Standards in Genomic Sciences 2016, 11: 72.
(5) Nováková E, Husník F, Šochová E, Hypša V: Arsenophonus and Sodalis symbionts in louse flies: an analogy to the Wigglesworthia and Sodalis system in tsetse flies. Applied and Environmental Microbiology 2015, 81(18): 6189-6199.
(4) Duncan RP, Husnik F, Van Leuven JT, Gilbert DG, Dávalos LM, McCutcheon JP, Wilson ACC: Dynamic recruitment of amino acid transporters to the insect/symbiont interface. Molecular Ecology 2014, 23(6): 1608-1623.
(3) Husnik F, Nikoh N, Koga R, Ross L, Duncan RP, Fujie M, Tanaka M, Satoh N, Bachtrog D, Wilson ACC, von Dohlen CD, Fukatsu T, McCutcheon JP: Horizontal Gene Transfer from Diverse Bacteria to an Insect Genome Enables a Tripartite Nested Mealybug Symbiosis. Cell 2013, 153(7): 1567-1578.