Seminar "Systems proteomics of liver mitochondrial activity" by Dr. Yibo Wu.

Speaker: Dr. Yibo Wu

Young Chief Investigator, RIKEN Center for Integrative Medical Sciences

Title: Systems proteomics of liver mitochondrial activity

Abstract:  The manner by which genotype and environment affect complex phenotypes is one of the fundamental questions in biology. Despite the progress in “omics” technologies, the majority of genetic factors influencing complex traits remain unknown. In this study, we examine mitochondrial links to liver metabolism through the genome, transcriptome, proteome, and metabolome of 386 individuals in the BXD mouse reference population. Several links were validated between genetic variants toward transcripts, proteins, metabolites, and phenotypes. Most significantly, we identified DHTKD1 as a novel diabetic gene that is shared in mice and humans. By applying SWATH-MS to total tissue lysate and enriched mitochondria (mitoSWATH-MS), we identified several novel mitochondrial proteins. Together, these tools have substantially contributed toward the understanding of complex systems.