Cell based high throughput screening systems to identify bio-active compounds that target specific cellular targets

Dear All,

Cell Signal Unit (Yamamoto Unit) would like to inform you of a seminar by Dr. Nobumoto Watanabe from RIKEN.

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Date: Wednesday, February 3, 2021

Time: 11:00-12:00

Venue: L4F01 (Lab4, Level F)  or

             ZOOM: https://oist.zoom.us/j/93421096458?pwd=TCt1QXhPOVRBaDhQWW9hc3VWb3NOQT09

             Meeting ID: 934 2109 6458

             *If you need "passcode", please send e-mail to "yuki.nakagawa[at]oist.jp".

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Speaker:

Dr. Nobumoto Watanabe, RIKEN

Title:
Cell based high throughput screening systems to identify bio-active compounds that target specific cellular targets

Abstract:
To isolate bio-active compounds that target the important cellular molecule, in vitro assay system to evaluate the effect of the compounds on the activity of the molecule is frequently used. However, we frequently experience that the identified compounds with potent activity in vitro does not work in the cell. The main reasons for this failure are the impermeability, insolubility or instability of the compounds in the cell system.    Therefore, it is important to identify the bio-active compounds that can be active in cell-based system. To identify such compounds that target a specific cellular target, we should establish cell-based screening system that specifically gives clear hit signals only when bio-active compounds specific for the cellular target are applied.

In this seminar, I will introduce several cell based high-throughput screening systems, which were developed in our group to identify small-molecule inhibitors of phosphorylation-dependent signaling pathway. I will also introduce a cell based screening system to identify ROS (reactive oxygen species) inducer. Several examples of bio-active compounds will be presented and the potential uses of this system are discussed.

Host:
Prof. Tadashi Yamamoto

We hope to see many of you at the seminar.

Best regards,

Yuki Nakagawa

Research Unit Administrator

Cell Signal Unit