A214
Course Coordinator: 
Yohei Yokobayashi
Nucleic Acid Chemistry and Engineering
Description: 

In this course, students will learn basic principles of nucleic acid chemistry and engineering through lectures and discussions. The students will then use the basic knowledge to deepen their understanding of the current research in the field of nucleic acid chemistry and engineering. Finally, the students will design, construct, and characterize functional nucleic acids in the laboratory while learning basic experimental skills to manipulate nucleic acids.

 
Aim: 
This course introduces the basic principles and current research in nucleic acid chemistry and engineering through lectures, discussions and laboratory sessions.
Detailed Syllabus: 

Basic nucleic acid chemistry (3 hr)

  1. ​Structure (DNA, RNA, unnatural nucleic acids, secondary/tertiary structures)
  2. Thermodynamics (hybridization)​

Synthesis of nucleic acids (4.5 hr)​​

  1. Chemical synthesis (solid phase synthesis)
  2. Biochemical synthesis (PCR, in vitro transcription, gene synthesis, biological synthesis, etc.)

Analysis of nucleic acids (4.5 hr)

  1. Chemical analysis (UV, electrophoresis, CD, nuclease probing, SHAPE, etc.)
  2. Sequence analysis (Sanger, Illumina, PacBio, nanopore, etc.)

Nucleic Acid Engineering (12 hr)

  • Synthetic nucleic acids
  1. Unnatural bases and backbones
  2. Self-assembly, materials
  3. Nucleic acid amplification and detection
  4. Therapeutics
  5. Aptamers
  6. Catalytic nucleic acids
  7. In vitro selection, in vitro evolution
  8. Molecular computation
  • Biological nucleic acids
  1. Riboswitches
  2. Ribozymes

Laboratory: Design, construction, and characterization of functional nucleic acids (12-16 hr labs)

Course Type: 
Elective
Credits: 
2
Assessment: 
Reports 40%; Presentations 40%; Exam 20%
Text Book: 
None
Reference Book: 
original papers will be supplied as required