Cancelled: [Seminar] "Force-sensitive actin polymerization systems" by Prof. Naoki Watanabe, Kyoto University Graduate School of Medicine
[Abstract] The actin network generates forces while receiving forces from outer environment. Within the network, actin polymerizes and disassembles continuously. I will present our findings of fast mechanotransduction systems such as actin filament regeneration and stabilization by Formin homology proteins (1-3) and the Brownian ratchet-based force sensor at the tip of thin lamellipodia (4), both of which would not be recognized without real-time monitoring of single-molecule dynamics. I also introduce a new-concept super-resolution microscopy IRIS, which delivers high-definition image with high-density labeling, potentially resolving the problem of inhomogeneous staining (‘Super-resolution Dilemma’), and enables unlimited multiple staining in a single specimen (5).
1. Higashida, C. et al. (2013) Nat. Cell Biol. 15: 395-405.
2. Yamashiro, S. et al. (2018) Mol. Biol. Cell 29: 1941-1947.
3. Mizuno, H. et al. (2018) Proc. Natl. Acad. Sci. USA, 115: E5000-E5007.
4. Koseki, K. et al. (2019) Genes Cells 24, 705-718.
5. Kiuchi, T. et al. (2015) Nat. Methods 212: 743-746.