Seminar by Prof. Shum "Towards induced pluripotent cell-derived sensory neurons for fate commitment of bone marrow stromal cell-derived Schwann cells and beyond."
Towards induced pluripotent cell-derived sensory neurons for fate commitment of bone marrow stromal cell-derived Schwann cells and beyond.
Our ultimate goal of in vitro derivation of Schwann cells (SchCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SchC-like cells. Co-culture of the SchC-like cells with embryonic dorsal root ganglion neurons (rat) facilitated contact-mediated signaling that accomplished the switch to fate-committed SCs. Microarray analysis and in vitro myelination provided evidence that the human BMSC-derived SchCs were functionally mature. This was reinforced by repair and myelination phenotypes observable in vivo with the derived SchCs seeded into a nerve guide as an implant across a critical gap in a rat model of sciatic nerve injury.