Garth Ilsley

The fundamental toolkit of proteins and other molecules are shared by every cell in an organism, but are used in different combinations at different times. One way these combinations are selected is via regulatory DNA called enhancers which interact with a class of proteins called transcription factors. These in turn affect which cellular tools are manufactured for further use in the cell. Since regulatory DNA sequence changes very little from cell to cell, every cell has the full set of instructions and yet is able to follow only the part that is relevant to its place in the organism.

The process of development from a fertilised egg to an adult is an amazing instance of this, and is the context in which I have developed models at the single-cell level in ​Drosophila​. 

In addition, I collaborate with the Luscombe Unit on a single-cell RNA-seq project in Ciona​, a marine chordate. 


Ilsley, G. R., Fisher, J., Apweiler, R., DePace, A. H. & Luscombe, N. M. Cellular resolution models for even skipped regulation in the entire Drosophila embryo. eLife 2, (2013).
Pubmed Journal

Crocker, J., Ilsley, G. R. & Stern, D. L. Quantitatively predictable control of Drosophila transcriptional enhancers in vivo with engineered transcription factors. Nat Genet 48, 292–298 (2016).
Pubmed Journal

Crocker, J. & Ilsley, G. R. Using synthetic biology to study gene regulatory evolution. Current Opinion in Genetics & Development 47, 91–101 (2017).
Pubmed Journal