NGS Platforms
We have been operating six next-generation sequencers (NGSs).
・Irys
NovaSeq6000
NovaSeq6000 system offers high-throughput sequencing across a broad range of applications. It meets needs with unprecedented throughput at the lowest cost per sample. We are going to start run/lane sharing service on NovaSeq6000 sequencing from October 1st. More information for NovaSeq6000 specification.
Miseq
MiSeq system is a benchtop sequencer optimized for speed and longer reads (maximum 2 x 300 paired-end reads using MiSeq Reagent Kit v3). Due to it's unique read length and throughput, MiSeq is a good instrument for data production for small genomes or amplicon sequencing projects. Since the library made for MiSeq is compatible with HiSeq, MiSeq is also used for testing library protocols before sequencing on the HiSeq. The v3 kit retains pre-filled, ready-to-use reagent cartiridge. More information for Miseq Specifications.
PacBio Sequel2
The Sequel II system uses ultra-long read sequences in tens of kb increments with no GC bias.
The Sequel II System allows you to capture large structural changes that have been difficult to achieve with short read sequencing.
-Highly accurate genome sequencing with dozens of kb long reads
-Over 100Gb of data can be acquired with a single cell
-Simultaneous analysis of multiple samples in a single cell (carpooling sequences)
MinION
MinION system is a single molecule, real-time DNA sequencing system that provides longest read lengths. We are going to start service on MinION sequencing from September 1st. More information for spec. of MinION at Full comparison table.
PromethION
PromethION system is a single molecule, real-time DNA sequencing system that provides the longest read lengths. The system will be installed in January, 2019. More information for spec. of PromethION at Full comparison table.
Irys
BioNano Irys system is acquire high-resolution, multi-color images and conduct single-molecule analysis of long DNA molecules with the automated, benchtop instrument. More information at Irys® Instrument.
IrysChip V2
Throughput (per chip) | Flowcell/Chip | Mapping Run Time |
---|---|---|
50-100 Gb | 2 | ~24 hrs |