Publications from the Yanagida lab at Kyoto University


  • Y. Shiroiwa , T. Hayashi , Y. Fujita , A. Villar-Briones , N. Ikai , K. Takeda , M. Ebe and M. Yanagida.,(2011) Mis17 Is a Regulatory Module of the Mis6-Mal2-Sim4 Centromere Complex That Is Required for the Recruitment of CenH3/CENP-A in Fission Yeast. PLoS One,6.,e17761[PubMed],[PLoS One]
  • Kiyomitsu, T. , Murakami, H. and Yanagida, M.(2011) Protein interaction domain mapping of human kinetochore protein Blinkin reveals a consensus motif for binding of spindle assembly checkpoint proteins Bub1 and BubR1. Molecular and Cellular Biology,5., 998-1011 [PubMed],[MCB]


  • Takahashi, K. , Imano, R. , Kibe, T. , Seimiya, H. , Muramatsu, Y. , Kawabata, N. , Tanaka, G. , Matsumoto, Y. , Hiromoto, T. , Koizumi, Y. , Nakazawa, N. , Yanagida, M. , Yukawa, M. , Tsuchiya, E. and Ueno, M., (2010)Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation. Molecular and Cellular Biology3.,495-506 [PubMed]. [MCB]
  • Yanagida M., (2010)Mostly DNA, a bit of glucose, and the next 50 years., Molecular biology of the cell21 ,3826 [PubMed][MBoC]
  • Kiyomitsu T, Iwasaki O, Obuse C, Yanagida M. (2010)Inner centromere formation requires hMis14, a trident kinetochore protein that specifically recruits HP1 to human chromosomes. J Cell Biol.188 , 791-807 ,[PubMed] ,[JCBrupress]
  • Tomas Pluskal, Takahiro Nakamura, Alejandro Villar-Briones and Mitsuhiro Yanagida.(2010)Metabolic profiling of the fission yeast S. pombe: quantification of compounds under different temperatures and genetic perturbationMol. BioSyst.6 182-198.,[RSCpublishing] ,[PubMed]


  • Yanagida M., (2009)Cellular quiescence: are controlling genes conserved? Trends Cell Biol.12,705-15 [PubMed]
  • Yanagida M., (2009)Clearing the way for mitosis: is cohesin a target? Nat Rev Mol Cell Biol. 10 ,489-96.[PubMed]
  • Tallada VA, Tanaka K, Yanagida M, Hagan IM.(2009)The S. pombe mitotic regulator Cut12 promotes spindle pole body activation and integration into the nuclear envelope. J Cell Biol. 185 ,875-88[PubMed]
  • Irvine DV, Goto DB, Vaughn MW, Nakaseko Y, McCombie WR, Yanagida M, Martienssen R. (2009)Mapping epigenetic mutations in fission yeast using whole-genome next-generation sequencing.Genome Res. 19 1077-83.[PubMed]
  • Hanyu Y, Imai KK, Kawasaki Y, Nakamura T, Nakaseko Y, Nagao K, Kokubu A, Ebe M, Fujisawa A, Hayashi T, Obuse C, Yanagida M.(2009)Schizosaccharomyces pombe cell division cycle under limited glucose requires Ssp1 kinase, the putative CaMKK, and Sds23, a PP2A-related phosphatase inhibitor.Genes to Cell.14 ,539 - 554[PubMed]
  • Sajiki K.,Hatanaka M.,Nakamura T.,Takeda K.,Shimanuki M.,Yoshida T.,Hanyu Y.,Hayashi T.,Nakaseko Y. and Yanagida M.(2009)Genetic control of cellular quiescence in S. pombe.J Cell Sci.122 ,1418-29.[PubMed]
  • Williams J.S.,Hayashi T.,Yanagida M. and Russell P.(2009)Fission yeast Scm3 mediates stable assembly of Cnp1/CENP-A into centromeric chromatin.Mol Cell.33 ,287-98.[PubMed]
  • Bolanos-Garcia V.M.,Kiyomitsu T.,D'Arcy S.,Chirgadze D.Y.,Grossmann J.G.,Matak-Vinkovic D.,Venkitaraman A.R.,Yanagida M.,Robinson C.V. and Blundell T.L.(2009)The crystal structure of the N-terminal region of BUB1 provides insight into the mechanism of BUB1 recruitment to kinetochores.Structure.17 ,105-16.[PubMed]


  • Nakazawa N.,Nakamura T.,Kokubu A.,Ebe M.,Nagao K. and Yanagida M.(2008)Dissection of the essential steps for condensin accumulation at kinetochores and rDNAs during fission yeast mitosis.J Cell Biol. 180 ,1115-31.[PubMed]
  • Kimata Y.,Matsuyama A.,Nagao K.,Furuya K.,Obuse C.,Yoshida M. and Yanagida M.(2008)Diminishing HDACs by drugs or mutations promotes normal or abnormal sister chromatid separation by affecting APC/C and adherin.J Cell Sci.121 ,1107-18.[PubMed]
  • Adachi Y.,Kokubu A.,Ebe M.,Nagao K. and Yanagida M.(2008)Cut1/separase-dependent roles of multiple phosphorylation of fission yeast cohesion subunit Rad21 in post-replicative damage repair and mitosis.Cell Cycle.7 ,765-76.[PubMed]
  • Yokoyama K.,Nakagawa M.,Satoh M.,Saitoh S.,Dohmae N.,Harada A.,Satoh N.,Karasawa K.,Takio K.,Yanagida M. and Inoue K.(2008)Expression of a novel 90-kDa protein, Lsd90, involved in the metabolism of very long-chain fatty acid-containing phospholipids in a mitosis-defective fission yeast mutant.J Biochem.143 ,369-75.[PubMed]


  • Kanoh J. and Yanagida M. Tel2: a common partner of PIK-related kinases and a link between DNA checkpoint and nutritional response? Genes Cells.12 ,1301-4. [PubMed]
  • Kiyomitsu T.,Obuse C. and Yanagida M.Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1. Dev Cell. 13 ,663-76. [PubMed]
  • Hayashi, T., Hatanaka, M., Nagao, K., Nakaseko, Y., Kanoh, J., Kokubu, A., Ebe, M. and Yanagida, M. (2007) Rapamycin-sensitivity of the S. pombe tor2 mutant and organization of two highly phosphorylated TOR complexes by specific and common subunits. Genes Cells12, 1357-70.[PubMed]
  • Fujita, Y., Hayashi, T., Kiyomitsu, T., Toyoda, Y., Kokubu, A., Obuse, C. and Yanagida, M. (2007) Priming of centromere for CENP-A recruitment by human hMis18α, hMis1β, and M18BP1. Dev Cell12, 17-30. [PubMed]
  • Murakami, H., Goto, D.B., Toda, T., Chen, E.S., Grewal, S.I., Martienssen, R.A. and Yanagida, M. (2007) Ribonuclease activity of Dis3 is required for mitotic progression and provides a possible link between heterochromatin and kinetochore function. PLoS ONE2, e317. [PubMed]
  • Shimanuki, M., Chung, S.Y., Chikashige, Y., Kawasaki, Y., Uehara, L., Tsutsumi, C., Hatanaka, M., Hiraoka, Y., Nagao, K. and Yanagida, M. (2007) Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombeGenes Cells12, 677-692. [PubMed]


  • Aoki, K., Nakaseko, Y., Kinoshita, K., Goshima, G. and Yanagida, M. (2006) CDC2 phosphorylation of the fission yeast dis1 ensures accurate chromosome segregation. Curr Biol16, 1627-1635. [PubMed]
  • Ikai, N. and Yanagida, M. (2006) Cdc48 is required for the stability of Cut1/separase in mitotic anaphase. J Struct Biol156, 50-61. [PubMed]
  • Kawasaki, Y., Nagao, K., Nakamura, T. and Yanagida, M. (2006) Fission yeast MAP kinase is required for the increased securin-separase interaction that rescues separase mutants under stresses. Cell Cycle5, 1831-1839. [PubMed]
  • Kumada, K., Yao, R., Kawaguchi, T., Karasawa, M., Hoshikawa, Y., Ichikawa, K., Sugitani, Y., Imoto, I., Inazawa, J., Sugawara, M., Yanagida, M. and Noda, T. (2006) The selective continued linkage of centromeres from mitosis to interphase in the absence of mammalian separase. J Cell Biol172, 835-846. [PubMed]
  • Maruyama, T., Nakamura, T., Hayashi, T. and Yanagida, M. (2006) Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation. EMBO J25, 2420-2431. [PubMed]
  • Mochida, S. and Yanagida, M. (2006) Distinct modes of DNA damage response in S. pombe G0 and vegetative cells. Genes Cells11, 13-27. [PubMed]
  • Nagao, K. and Yanagida, M. (2006) Securin can have a separase cleavage site by substitution mutations in the domain required for stabilization and inhibition of separase. Genes Cells11, 247-260. [PubMed]
  • Takeda, K. and Yanagida, M. (2006) [How does fission yeast cut8 regulate the nuclear localization of proteasome?]. Tanpakushitsu Kakusan Koso51, 1241-1244. [PubMed]
  • Toyoda, Y. and Yanagida, M. (2006) Coordinated Requirements of Human Topo II and Cohesin for Metaphase Centromere Alignment under Mad2-dependent Spindle Checkpoint Surveillance. Mol Biol Cell[PubMed]


  • Takeda, K. and Yanagida, M. (2005) Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8. Cell122, 393-405. [PubMed]
  • Yanagida, M. (2005) Basic mechanism of eukaryotic chromosome segregation. Philos Trans R Soc Lond B Biol Sci360, 609-621. [PubMed]
  • Yanagida, M. and Nasmyth, K. (2005) Introduction: dissection and simplification of chromosome segregation. Philos Trans R Soc Lond B Biol Sci360, 481. [PubMed]


  • Chen, E.S., Sutani, T. and Yanagida, M. (2004) Cti1/C1D interacts with condensin SMC hinge and supports the DNA repair function of condensin. Proc Natl Acad Sci U S A101, 8078-8083. [PubMed]
  • Hayashi, T., Fujita, Y., Iwasaki, O., Adachi, Y., Takahashi, K. and Yanagida, M. (2004) Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell118, 715-729. [PubMed]
  • Kimata, Y. and Yanagida, M. (2004) Suppression of a mitotic mutant by tRNA-Ala anticodon mutations that produce a dominant defect in late mitosis. J Cell Sci117, 2283-2293. [PubMed]
  • Mochida, S., Esashi, F., Aono, N., Tamai, K., O'Connell, M.J. and Yanagida, M. (2004) Regulation of checkpoint kinases through dynamic interaction with Crb2. EMBO J23, 418-428. [PubMed]
  • Nagao, K., Adachi, Y. and Yanagida, M. (2004) Separase-mediated cleavage of cohesin at interphase is required for DNA repair. Nature430, 1044-1048. [PubMed]
  • Obuse, C., Iwasaki, O., Kiyomitsu, T., Goshima, G., Toyoda, Y. and Yanagida, M. (2004) A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol6, 1135-1141. [PubMed]
  • Yanagida, M. (2004) Heads and tails. Nature429, 135. [PubMed]
  • Yuasa, T., Hayashi, T., Ikai, N., Katayama, T., Aoki, K., Obara, T., Toyoda, Y., Maruyama, T., Kitagawa, D., Takahashi, K., Nagao, K., Nakaseko, Y. and Yanagida, M. (2004) An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation. Genes Cells9, 1069-1082. [PubMed]


  • Chen, E.S., Saitoh, S., Yanagida, M. and Takahashi, K. (2003) A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast. Mol Cell11, 175-187. [PubMed]
  • Chen, E.S., Yanagida, M. and Takahashi, K. (2003) Does a GATA factor make the bed for centromeric nucleosomes? Cell Cycle2, 277-278. [PubMed]
  • Goshima, G., Iwasaki, O., Obuse, C. and Yanagida, M. (2003) The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore. EMBO J22, 2752-2763. [PubMed]
  • Goshima, G., Kiyomitsu, T., Yoda, K. and Yanagida, M. (2003) Human centromere chromatin protein hMis12, essential for equal segregation, is independent of CENP-A loading pathway. J Cell Biol160, 25-39. [PubMed]
  • Matsumura, T., Yuasa, T., Hayashi, T., Obara, T., Kimata, Y. and Yanagida, M. (2003) A brute force postgenome approach to identify temperature-sensitive mutations that negatively interact with separase and securin plasmids. Genes Cells8, 341-355. [PubMed]
  • Sakai, A., Hizume, K., Sutani, T., Takeyasu, K. and Yanagida, M. (2003) Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein-protein assembly. EMBO J22, 2764-2775. [PubMed]
  • Yanagida, M. (2003) [Studies on the structure of double-helix DNA in the last half century: My study of DNA]. Tanpakushitsu Kakusan Koso48, 690-692. [PubMed]


  • Aono, N., Sutani, T., Tomonaga, T., Mochida, S. and Yanagida, M. (2002) Cnd2 has dual roles in mitotic condensation and interphase. Nature417, 197-202. [PubMed]
  • Nagao, K. and Yanagida, M. (2002) Regulating sister chromatid separation by separase phosphorylation. Dev Cell2, 2-4. [PubMed]
  • Nakamura, T., Nagao, K., Nakaseko, Y. and Yanagida, M. (2002) Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation. Genes Cells7, 1113-1124. [PubMed]
  • Toyoda, Y., Furuya, K., Goshima, G., Nagao, K., Takahashi, K. and Yanagida, M. (2002) Requirement of chromatid cohesion proteins rad21/scc1 and mis4/scc2 for normal spindle-kinetochore interaction in fission yeast. Curr Biol12, 347-358. [PubMed]
  • Yanagida, M. (2002) The model unicellular eukaryote, Schizosaccharomyces pombeGenome Biol3, COMMENT2003. [PubMed]
  • Yoshimura, S.H., Hizume, K., Murakami, A., Sutani, T., Takeyasu, K. and Yanagida, M. (2002) Condensin architecture and interaction with DNA: regulatory non-SMC subunits bind to the head of SMC heterodimer. Curr Biol12, 508-513. [PubMed]


  • Goshima, G. and Yanagida, M. (2001) Time course analysis of precocious separation of sister centromeres in budding yeast: continuously separated or frequently reassociated? Genes Cells6, 765-773. [PubMed]
  • Morishita, J., Matsusaka, T., Goshima, G., Nakamura, T., Tatebe, H. and Yanagida, M. (2001) Bir1/Cut17 moving from chromosome to spindle upon the loss of cohesion is required for condensation, spindle elongation and repair. Genes Cells6, 743-763. [PubMed]
  • Nakaseko, Y., Goshima, G., Morishita, J. and Yanagida, M. (2001) M phase-specific kinetochore proteins in fission yeast: microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase. Curr Biol11, 537-549. [PubMed]
  • Tatebe, H., Goshima, G., Takeda, K., Nakagawa, T., Kinoshita, K. and Yanagida, M. (2001) Fission yeast living mitosis visualized by GFP-tagged gene products. Micron32, 67-74. [PubMed]


  • Esashi, F., Mochida, S., Matsusaka, T., Obara, T., Ogawa, A., Tamai, K. and Yanagida, M. (2000) Establishment of and recovery from damage checkpoint requires sequential interactions of Crb2 with protein kinases Rad3, Chk1, and Cdc2. Cold Spring Harb Symp Quant Biol65, 443-449. [PubMed]
  • Goshima, G. and Yanagida, M. (2000) Establishing biorientation occurs with precocious separation of the sister kinetochores, but not the arms, in the early spindle of budding yeast. Cell100, 619-633. [PubMed]
  • Kondoh, H., Yuasa, T. and Yanagida, M. (2000) Mis3 with a conserved RNA binding motif is essential for ribosome biogenesis and implicated in the start of cell growth and S phase checkpoint. Genes Cells5, 525-541. [PubMed]
  • Takahashi, K., Chen, E.S. and Yanagida, M. (2000) Requirement of Mis6 centromere connector for localizing a CENP-A-like protein in fission yeast. Science288, 2215-2219. [PubMed]
  • Takahashi, K., Saitoh, S. and Yanagida, M. (2000) Application of the chromatin immunoprecipitation method to identify in vivo protein-DNA associations in fission yeast. Sci STKE2000, PL1. [PubMed]
  • Takahashi, K. and Yanagida, M. (2000) Cell cycle. Replication meets cohesion. Science289, 735-736. [PubMed]
  • Tanaka, K., Yonekawa, T., Kawasaki, Y., Kai, M., Furuya, K., Iwasaki, M., Murakami, H., Yanagida, M. and Okayama, H. (2000) Fission yeast Eso1p is required for establishing sister chromatid cohesion during S phase. Mol Cell Biol20, 3459-3469. [PubMed]
  • Tatebe, H. and Yanagida, M. (2000) Cut8, essential for anaphase, controls localization of 26S proteasome, facilitating destruction of cyclin and Cut2. Curr Biol10, 1329-1338. [PubMed]
  • Tomonaga, T., Nagao, K., Kawasaki, Y., Furuya, K., Murakami, A., Morishita, J., Yuasa, T., Sutani, T., Kearsey, S.E., Uhlmann, F., Nasmyth, K. and Yanagida, M. (2000) Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase. Genes Dev14, 2757-2770. [PubMed]
  • Yanagida, M. (2000) Cell cycle mechanisms of sister chromatid separation; roles of Cut1/separin and Cut2/securin. Genes Cells5, 1-8. [PubMed]


  • Esashi, F. and Yanagida, M. (1999) Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpoint. Mol Cell4, 167-174. [PubMed]
  • Goshima, G., Saitoh, S. and Yanagida, M. (1999) Proper metaphase spindle length is determined by centromere proteins Mis12 and Mis6 required for faithful chromosome segregation. Genes Dev13, 1664-1677. [PubMed]
  • Nakaseko, Y. and Yanagida, M. (1999) [Fission yeast mutants defective in sisiter chromatid separation]. Tanpakushitsu Kakusan Koso44, 1703-1710. [PubMed]
  • Sutani, T., Yuasa, T., Tomonaga, T., Dohmae, N., Takio, K. and Yanagida, M. (1999) Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4. Genes Dev13, 2271-2283. [PubMed]
  • Watanabe, M., Fukuda, M., Yoshida, M., Yanagida, M. and Nishida, E. (1999) Involvement of CRM1, a nuclear export receptor, in mRNA export in mammalian cells and fission yeast. Genes Cells4, 291-297. [PubMed]
  • Yamashita, Y.M., Nakaseko, Y., Kumada, K., Nakagawa, T. and Yanagida, M. (1999) Fission yeast APC/cyclosome subunits, Cut20/Apc4 and Cut23/Apc8, in regulating metaphase-anaphase progression and cellular stress responses. Genes Cells4, 445-463. [PubMed]
  • Yanagida, M. (1999) From phage to chromosome biology: a personal account. J Mol Biol293, 181-185. [PubMed]
  • Yanagida, M., Yamashita, Y.M., Tatebe, H., Ishii, K., Kumada, K. and Nakaseko, Y. (1999) Control of metaphase-anaphase progression by proteolysis: cyclosome function regulated by the protein kinase A pathway, ubiquitination and localization. Philos Trans R Soc Lond B Biol Sci354, 1559-1569; discussion 1569-1570. [PubMed]


  • Furuya, K., Takahashi, K. and Yanagida, M. (1998) Faithful anaphase is ensured by Mis4, a sister chromatid cohesion molecule required in S phase and not destroyed in G1 phase. Genes Dev12, 3408-3418. [PubMed]
  • Ishii, K. and Yanagida, M. (1998) [Control of M-phase progression by protein phosphatases]. Tanpakushitsu Kakusan Koso43, 987-995. [PubMed]
  • Kumada, K., Nakamura, T., Nagao, K., Funabiki, H., Nakagawa, T. and Yanagida, M. (1998) Cut1 is loaded onto the spindle by binding to Cut2 and promotes anaphase spindle movement upon Cut2 proteolysis. Curr Biol8, 633-641. [PubMed]
  • Matsusaka, T., Imamoto, N., Yoneda, Y. and Yanagida, M. (1998) Mutations in fission yeast Cut15, an importin alpha homolog, lead to mitotic progression without chromosome condensation. Curr Biol8, 1031-1034. [PubMed]
  • Nabeshima, K., Nakagawa, T., Straight, A.F., Murray, A., Chikashige, Y., Yamashita, Y.M., Hiraoka, Y. and Yanagida, M. (1998) Dynamics of centromeres during metaphase-anaphase transition in fission yeast: Dis1 is implicated in force balance in metaphase bipolar spindle. Mol Biol Cell9, 3211-3225. [PubMed]
  • Yanagida, M. (1998) Finding your theme. Curr Biol8, R861. [PubMed]
  • Yanagida, M. (1998) Fission yeast cut mutations revisited: control of anaphase. Trends Cell Biol8, 144-149. [PubMed]
  • Yanagida, M. (1998) Broadening your horizons. Curr Biol8, R185. [PubMed]


  • Adachi, Y., Usukura, J. and Yanagida, M. (1997) A globular complex formation by Nda1 and the other five members of the MCM protein family in fission yeast. Genes Cells2, 467-479. [PubMed]
  • Fukuda, M., Asano, S., Nakamura, T., Adachi, M., Yoshida, M., Yanagida, M. and Nishida, E. (1997) CRM1 is responsible for intracellular transport mediated by the nuclear export signal. Nature390, 308-311. [PubMed]
  • Funabiki, H., Yamano, H., Nagao, K., Tanaka, H., Yasuda, H., Hunt, T. and Yanagida, M. (1997) Fission yeast Cut2 required for anaphase has two destruction boxes. EMBO J16, 5977-5987. [PubMed]
  • Hagan, I. and Yanagida, M. (1997) Evidence for cell cycle-specific, spindle pole body-mediated, nuclear positioning in the fission yeast Schizosaccharomyces pombe. J Cell Sci110 ( Pt 16), 1851-1866. [PubMed]
  • Nabeshima, K., Saitoh, S. and Yanagida, M. (1997) Use of green fluorescent protein for intracellular protein localization in living fission yeast cells. Methods Enzymol283, 459-471. [PubMed]
  • Nakaseko, Y. and Yanagida, M. (1997) A telomerase mutant defective in sister chromatid separation at mitosis. Bioessays19, 557-559. [PubMed]
  • Saitoh, S., Takahashi, K. and Yanagida, M. (1997) Mis6, a fission yeast inner centromere protein, acts during G1/S and forms specialized chromatin required for equal segregation. Cell90, 131-143. [PubMed]
  • Saka, Y., Esashi, F., Matsusaka, T., Mochida, S. and Yanagida, M. (1997) Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1. Genes Dev11, 3387-3400. [PubMed]
  • Sutani, T. and Yanagida, M. (1997) DNA renaturation activity of the SMC complex implicated in chromosome condensation. Nature388, 798-801. [PubMed]
  • Yamada, H., Kumada, K. and Yanagida, M. (1997) Distinct subunit functions and cell cycle regulated phosphorylation of 20S APC/cyclosome required for anaphase in fission yeast. J Cell Sci110 ( Pt 15), 1793-1804. [PubMed]
  • Yanagida, M. (1997) The experimental experience. Curr Biol7, R597. [PubMed]


  • Funabiki, H., Kumada, K. and Yanagida, M. (1996) Fission yeast Cut1 and Cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes. EMBO J15, 6617-6628. [PubMed]
  • Funabiki, H., Yamano, H., Kumada, K., Nagao, K., Hunt, T. and Yanagida, M. (1996) Cut2 proteolysis required for sister-chromatid seperation in fission yeast. Nature381, 438-441. [PubMed]
  • Ishii, K., Kumada, K., Toda, T. and Yanagida, M. (1996) Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+. EMBO J15, 6629-6640. [PubMed]
  • Kinoshita, K., Nemoto, T., Nabeshima, K., Kondoh, H., Niwa, H. and Yanagida, M. (1996) The regulatory subunits of fission yeast protein phosphatase 2A (PP2A) affect cell morphogenesis, cell wall synthesis and cytokinesis. Genes Cells1, 29-45. [PubMed]
  • Kumada, K., Yanagida, M. and Toda, T. (1996) Caffeine-resistance in fission yeast is caused by mutations in a single essential gene, crm1+. Mol Gen Genet250, 59-68. [PubMed]
  • Nakaseko, Y., Nabeshima, K., Kinoshita, K. and Yanagida, M. (1996) Dissection of fission yeast microtubule associating protein p93Dis1: regions implicated in regulated localization and microtubule interaction. Genes Cells1, 633-644. [PubMed]
  • Okishio, N., Adachi, Y. and Yanagida, M. (1996) Fission yeast Nda1 and Nda4, MCM homologs required for DNA replication, are constitutive nuclear proteins. J Cell Sci109 ( Pt 2), 319-326. [PubMed]
  • Saitoh, S., Takahashi, K., Nabeshima, K., Yamashita, Y., Nakaseko, Y., Hirata, A. and Yanagida, M. (1996) Aberrant mitosis in fission yeast mutants defective in fatty acid synthetase and acetyl CoA carboxylase. J Cell Biol134, 949-961. [PubMed]
  • Su, S.S., Tanaka, Y., Samejima, I., Tanaka, K. and Yanagida, M. (1996) A nitrogen starvation-induced dormant G0 state in fission yeast: the establishment from uncommitted G1 state and its delay for return to proliferation. J Cell Sci109 ( Pt 6), 1347-1357. [PubMed]
  • Yamashita, Y.M., Nakaseko, Y., Samejima, I., Kumada, K., Yamada, H., Michaelson, D. and Yanagida, M. (1996) 20S cyclosome complex formation and proteolytic activity inhibited by the cAMP/PKA pathway. Nature384, 276-279. [PubMed]


  • Hagan, I. and Yanagida, M. (1995) The product of the spindle formation gene sad1+ associates with the fission yeast spindle pole body and is essential for viability. J Cell Biol129, 1033-1047. [PubMed]
  • Kumada, K., Su, S., Yanagida, M. and Toda, T. (1995) Fission yeast TPR-family protein nuc2 is required for G1-arrest upon nitrogen starvation and is an inhibitor of septum formation. J Cell Sci108 ( Pt 3), 895-905. [PubMed]
  • Matsusaka, T., Hirata, D., Yanagida, M. and Toda, T. (1995) A novel protein kinase gene ssp1+ is required for alteration of growth polarity and actin localization in fission yeast. EMBO J14, 3325-3338. [PubMed]
  • Murakami, S., Yanagida, M. and Niwa, O. (1995) A large circular minichromosome of Schizosaccharomyces pombe requires a high dose of type II DNA topoisomerase for its stabilization. Mol Gen Genet246, 671-679. [PubMed]
  • Nabeshima, K., Kurooka, H., Takeuchi, M., Kinoshita, K., Nakaseko, Y. and Yanagida, M. (1995) p93dis1, which is required for sister chromatid separation, is a novel microtubule and spindle pole body-associating protein phosphorylated at the Cdc2 target sites. Genes Dev9, 1572-1585. [PubMed]
  • Shimanuki, M., Saka, Y., Yanagida, M. and Toda, T. (1995) A novel essential fission yeast gene pad1+ positively regulates pap1+-dependent transcription and is implicated in the maintenance of chromosome structure. J Cell Sci108 (Pt 2), 569-579. [PubMed]
  • Yanagida, M. (1995) Frontier questions about sister chromatid separation in anaphase. Bioessays17, 519-526. [PubMed]


  • Chikashige, Y., Ding, D.Q., Funabiki, H., Haraguchi, T., Mashiko, S., Yanagida, M. and Hiraoka, Y. (1994) Telomere-led premeiotic chromosome movement in fission yeast. Science264, 270-273. [PubMed]
  • Saka, Y., Fantes, P., Sutani, T., McInerny, C., Creanor, J. and Yanagida, M. (1994) Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control. EMBO J13, 5319-5329. [PubMed]
  • Saka, Y., Fantes, P. and Yanagida, M. (1994) Coupling of DNA replication and mitosis by fission yeast rad4/cut5. J Cell Sci Suppl18, 57-61. [PubMed]
  • Saka, Y., Sutani, T., Yamashita, Y., Saitoh, S., Takeuchi, M., Nakaseko, Y. and Yanagida, M. (1994) Fission yeast cut3 and cut14, members of a ubiquitous protein family, are required for chromosome condensation and segregation in mitosis. EMBO J13, 4938-4952. [PubMed]
  • Samejima, I. and Yanagida, M. (1994) Bypassing anaphase by fission yeast cut9 mutation: requirement of cut9+ to initiate anaphase. J Cell Biol127, 1655-1670. [PubMed]
  • Samejima, I. and Yanagida, M. (1994) Identification of cut8+ and cek1+, a novel protein kinase gene, which complement a fission yeast mutation that blocks anaphase. Mol Cell Biol14, 6361-6371. [PubMed]
  • Takahashi, K., Yamada, H. and Yanagida, M. (1994) Fission yeast minichromosome loss mutants mis cause lethal aneuploidy and replication abnormality. Mol Biol Cell5, 1145-1158. [PubMed]
  • Yamano, H., Ishii, K. and Yanagida, M. (1994) Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. EMBO J13, 5310-5318. [PubMed]
  • Yoshida, T., Toda, T. and Yanagida, M. (1994) A calcineurin-like gene ppb1+ in fission yeast: mutant defects in cytokinesis, cell polarity, mating and spindle pole body positioning. J Cell Sci107 ( Pt 7), 1725-1735. [PubMed]


  • Funabiki, H., Hagan, I., Uzawa, S. and Yanagida, M. (1993) Cell cycle-dependent specific positioning and clustering of centromeres and telomeres in fission yeast. J Cell Biol121, 961-976. [PubMed]
  • Kinoshita, N., Yamano, H., Niwa, H., Yoshida, T. and Yanagida, M. (1993) Negative regulation of mitosis by the fission yeast protein phosphatase ppa2. Genes Dev7, 1059-1071. [PubMed]
  • Miyake, S., Okishio, N., Samejima, I., Hiraoka, Y., Toda, T., Saitoh, I. and Yanagida, M. (1993) Fission yeast genes nda1+ and nda4+, mutations of which lead to S-phase block, chromatin alteration and Ca2+ suppression, are members of the CDC46/MCM2 family. Mol Biol Cell4, 1003-1015. [PubMed]
  • Mizukami, T., Chang, W.I., Garkavtsev, I., Kaplan, N., Lombardi, D., Matsumoto, T., Niwa, O., Kounosu, A., Yanagida, M., Marr, T.G. and et al. (1993) A 13 kb resolution cosmid map of the 14 Mb fission yeast genome by nonrandom sequence-tagged site mapping. Cell73, 121-132.[PubMed]
  • Mizukami, T., Garkavtsev, I., Beach, D., Marr, T., Niwa, O. and Yanagida, M. (1993) [Genome mapping of Schizosaccharomyces pombe]. Tanpakushitsu Kakusan Koso38, 677-684. [PubMed]
  • Saka, Y. and Yanagida, M. (1993) Fission yeast cut5+, required for S phase onset and M phase restraint, is identical to the radiation-damage repair gene rad4+Cell74, 383-393. [PubMed]
  • Samejima, I., Matsumoto, T., Nakaseko, Y., Beach, D. and Yanagida, M. (1993) Identification of seven new cut genes involved in Schizosaccharomyces pombe mitosis. J Cell Sci105 ( Pt 1), 135-143. [PubMed]
  • Shimanuki, M., Kinoshita, N., Ohkura, H., Yoshida, T., Toda, T. and Yanagida, M. (1993) Isolation and characterization of the fission yeast protein phosphatase gene ppe1+ involved in cell shape control and mitosis. Mol Biol Cell4, 303-313. [PubMed]
  • Stone, E.M., Yamano, H., Kinoshita, N. and Yanagida, M. (1993) Mitotic regulation of protein phosphatases by the fission yeast sds22 protein. Curr Biol3, 13-26. [PubMed]
  • Takeuchi, M. and Yanagida, M. (1993) A mitotic role for a novel fission yeast protein kinase dsk1 with cell cycle stage dependent phosphorylation and localization. Mol Biol Cell4, 247-260. [PubMed]
  • Toda, T., Shimanuki, M. and Yanagida, M. (1993) Two novel protein kinase C-related genes of fission yeast are essential for cell viability and implicated in cell shape control. EMBO J12, 1987-1995. [PubMed]


  • Hagan, I. and Yanagida, M. (1992) Kinesin-related cut7 protein associates with mitotic and meiotic spindles in fission yeast. Nature356, 74-76. [PubMed]
  • Shimanuki, M., Goebl, M., Yanagida, M. and Toda, T. (1992) Fission yeast sts1+ gene encodes a protein similar to the chicken lamin B receptor and is implicated in pleiotropic drug-sensitivity, divalent cation-sensitivity, and osmoregulation. Mol Biol Cell3, 263-273. [PubMed]
  • Shiozaki, K. and Yanagida, M. (1992) Functional dissection of the phosphorylated termini of fission yeast DNA topoisomerase II. J Cell Biol119, 1023-1036. [PubMed]
  • Takahashi, K., Murakami, S., Chikashige, Y., Funabiki, H., Niwa, O. and Yanagida, M. (1992) A low copy number central sequence with strict symmetry and unusual chromatin structure in fission yeast centromere. Mol Biol Cell3, 819-835. [PubMed]
  • Toda, T., Shimanuki, M., Saka, Y., Yamano, H., Adachi, Y., Shirakawa, M., Kyogoku, Y. and Yanagida, M. (1992) Fission yeast pap1-dependent transcription is negatively regulated by an essential nuclear protein, crm1. Mol Cell Biol12, 5474-5484. [PubMed]
  • Uzawa, S. and Yanagida, M. (1992) Visualization of centromeric and nucleolar DNA in fission yeast by fluorescence in situ hybridization. J Cell Sci101 ( Pt 2), 267-275. [PubMed]
  • Yanagida, M., Kinoshita, N., Stone, E.M. and Yamano, H. (1992) Protein phosphatases and cell division cycle control. Ciba Found Symp170, 130-140; discussion 140-136. [PubMed]


  • Horio, T., Uzawa, S., Jung, M.K., Oakley, B.R., Tanaka, K. and Yanagida, M. (1991) The fission yeast gamma-tubulin is essential for mitosis and is localized at microtubule organizing centers. J Cell Sci99 (Pt 4), 693-700. [PubMed]
  • Kinoshita, N., Goebl, M. and Yanagida, M. (1991) The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol11, 5839-5847. [PubMed]
  • Kinoshita, N., Yamano, H., Le Bouffant-Sladeczek, F., Kurooka, H., Ohkura, H., Stone, E.M., Takeuchi, M., Toda, T., Yoshida, T. and Yanagida, M. (1991) Sister-chromatid separation and protein dephosphorylation in mitosis. Cold Spring Harb Symp Quant Biol56, 621-628.[PubMed]
  • Murakami, S., Matsumoto, T., Niwa, O. and Yanagida, M. (1991) Structure of the fission yeast centromere cen3: direct analysis of the reiterated inverted region. Chromosoma101, 214-221. [PubMed]
  • Ohkura, H. and Yanagida, M. (1991) S. pombe gene sds22+ essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1. Cell64, 149-157. [PubMed]
  • Shiozaki, K. and Yanagida, M. (1991) A functional 125-kDa core polypeptide of fission yeast DNA topoisomerase II. Mol Cell Biol11, 6093-6102. [PubMed]
  • Takahashi, K., Murakami, S., Chikashige, Y., Niwa, O. and Yanagida, M. (1991) A large number of tRNA genes are symmetrically located in fission yeast centromeres. J Mol Biol218, 13-17. [PubMed]
  • Toda, T., Shimanuki, M. and Yanagida, M. (1991) Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases. Genes Dev5, 60-73. [PubMed]
  • Yanagida, M., Yamano, H., Stone, E.M., Kinoshita, N., Yoshida, T. and Shiozaki, K. (1991) Protein phosphatases in cell division: how vital are they? Princess Takamatsu Symp22, 137-144. [PubMed]


  • Hagan, I. and Yanagida, M. (1990) Novel potential mitotic motor protein encoded by the fission yeast cut7+ gene. Nature347, 563-566. [PubMed]
  • Hirano, T., Kinoshita, N., Morikawa, K. and Yanagida, M. (1990) Snap helix with knob and hole: essential repeats in S. pombe nuclear protein nuc2+Cell60, 319-328. [PubMed]
  • Kanbe, T., Hiraoka, Y., Tanaka, K. and Yanagida, M. (1990) The transition of cells of the fission yeast beta-tubulin mutant nda3-311 as seen by freeze-substitution electron microscopy. Requirement of functional tubulin for spindle pole body duplication. J Cell Sci96 ( Pt 2), 275-282.[PubMed]
  • Kinoshita, N., Ohkura, H. and Yanagida, M. (1990) Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell63, 405-415. [PubMed]
  • Masuda, H., Hirano, T., Yanagida, M. and Cande, W.Z. (1990) In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells. J Cell Biol110, 417-425. [PubMed]
  • Uzawa, S., Samejima, I., Hirano, T., Tanaka, K. and Yanagida, M. (1990) The fission yeast cut1+ gene regulates spindle pole body duplication and has homology to the budding yeast ESP1 gene. Cell62, 913-925. [PubMed]
  • Yanagida, M. (1990) Higher-order chromosome structure in yeast. J Cell Sci96 (Pt 1), 1-3. [PubMed]


  • Adachi, Y. and Yanagida, M. (1989) Higher order chromosome structure is affected by cold-sensitive mutations in a Schizosaccharomyces pombe gene crm1+ which encodes a 115-kD protein preferentially localized in the nucleus and its periphery. J Cell Biol108, 1195-1207. [PubMed]
  • Chikashige, Y., Kinoshita, N., Nakaseko, Y., Matsumoto, T., Murakami, S., Niwa, O. and Yanagida, M. (1989) Composite motifs and repeat symmetry in S. pombe centromeres: direct analysis by integration of NotI restriction sites. Cell57, 739-751. [PubMed]
  • Hirano, T., Konoha, G., Toda, T. and Yanagida, M. (1989) Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus. J Cell Biol108, 243-253. [PubMed]
  • Niwa, O., Matsumoto, T., Chikashige, Y. and Yanagida, M. (1989) Characterization of Schizosaccharomyces pombe minichromosome deletion derivatives and a functional allocation of their centromere. EMBO J8, 3045-3052. [PubMed]
  • Ohkura, H., Kinoshita, N., Miyatani, S., Toda, T. and Yanagida, M. (1989) The fission yeast dis2+ gene required for chromosome disjoining encodes one of two putative type 1 protein phosphatases. Cell57, 997-1007. [PubMed]
  • Yanagida, M. (1989) Gene products required for chromosome separation. J Cell Sci Suppl12, 213-229. [PubMed]


  • Hirano, T., Hiraoka, Y. and Yanagida, M. (1988) A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase. J Cell Biol106, 1171-1183. [PubMed]
  • Ohkura, H., Adachi, Y., Kinoshita, N., Niwa, O., Toda, T. and Yanagida, M. (1988) Cold-sensitive and caffeine-supersensitive mutants of the Schizosaccharomyces pombe dis genes implicated in sister chromatid separation during mitosis. EMBO J7, 1465-1473. [PubMed]


  • Matsumoto, S., Yanagida, M. and Nurse, P. (1987) Histone transcription in cell cycle mutants of fission yeast. EMBO J6, 1093-1097. [PubMed]
  • Matsumoto, T., Fukui, K., Niwa, O., Sugawara, N., Szostak, J.W. and Yanagida, M. (1987) Identification of healed terminal DNA fragments in linear minichromosomes of Schizosaccharomyces pombeMol Cell Biol7, 4424-4430. [PubMed]
  • Nakaseko, Y., Kinoshita, N. and Yanagida, M. (1987) A novel sequence common to the centromere regions of Schizosaccharomyces pombe chromosomes. Nucleic Acids Res15, 4705-4715. [PubMed]
  • Uemura, T., Morino, K., Uzawa, S., Shiozaki, K. and Yanagida, M. (1987) Cloning and sequencing of Schizosaccharomyces pombe DNA topoisomerase I gene, and effect of gene disruption. Nucleic Acids Res15, 9727-9739. [PubMed]
  • Uemura, T., Ohkura, H., Adachi, Y., Morino, K., Shiozaki, K. and Yanagida, M. (1987) DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombeCell50, 917-925. [PubMed]
  • Yanagida, M. (1987) Yeast tubulin genes. Microbiol Sci4, 115-118. [PubMed]


  • Adachi, Y., Toda, T., Niwa, O. and Yanagida, M. (1986) Differential expressions of essential and nonessential alpha-tubulin genes in Schizosaccharomyces pombeMol Cell Biol6, 2168-2178. [PubMed]
  • Hirano, T., Funahashi, S.I., Uemura, T. and Yanagida, M. (1986) Isolation and characterization of Schizosaccharomyces pombe cutmutants that block nuclear division but not cytokinesis. EMBO J5, 2973-2979. [PubMed]
  • Nakaseko, Y., Adachi, Y., Funahashi, S., Niwa, O. and Yanagida, M. (1986) Chromosome walking shows a highly homologous repetitive sequence present in all the centromere regions of fission yeast. EMBO J5, 1011-1021. [PubMed]
  • Uemura, T., Morikawa, K. and Yanagida, M. (1986) The nucleotide sequence of the fission yeast DNA topoisomerase II gene: structural and functional relationships to other DNA topoisomerases. EMBO J5, 2355-2361. [PubMed]


  • Asakura, Y., Kikuchi, Y. and Yanagida, M. (1985) A cruciform in the direct repeats of the yeast 2 micron DNA: Selective S1 nuclease cleavage at one of the three homologous palindromes. J Biochem (Tokyo)98, 41-47. [PubMed]
  • Matsumoto, S. and Yanagida, M. (1985) Histone gene organization of fission yeast: a common upstream sequence. EMBO J4, 3531-3538. [PubMed]


  • Hiraoka, Y., Toda, T. and Yanagida, M. (1984) The NDA3 gene of fission yeast encodes beta-tubulin: a cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis. Cell39, 349-358. [PubMed]
  • Nakaseko, Y., Niwa, O. and Yanagida, M. (1984) A meiotic mutant of the fission yeast Schizosaccharomyces pombe that produces mature asci containing two diploid spores. J Bacteriol157, 334-336. [PubMed]
  • Toda, T., Adachi, Y., Hiraoka, Y. and Yanagida, M. (1984) Identification of the pleiotropic cell division cycle gene NDA2 as one of two different alpha-tubulin genes in Schizosaccharomyces pombeCell37, 233-242. [PubMed]
  • Uemura, T. and Yanagida, M. (1984) Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization. EMBO J3, 1737-1744. [PubMed]
  • Yanagida, M. and Hiraoka, Y. (1984) [Dynamic structures of DNA, chromatin and chromosomes studied by video fluorescence microscopy]. Tanpakushitsu Kakusan Koso29, 329-343. [PubMed]
  • Yanagida, M., Suzuki, Y. and Toda, T. (1984) Molecular organization of the head of bacteriophage Teven: underlying design principles. Adv Biophys17, 97-146. [PubMed]


  • Toda, T., Umesono, K., Hirata, A. and Yanagida, M. (1983) Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombeJ Mol Biol168, 251-270. [PubMed]
  • Umesono, K., Toda, T., Hayashi, S. and Yanagida, M. (1983) Cell division cycle genes nda2 and nda3 of the fission yeast Schizosaccharomyces pombe control microtubular organization and sensitivity to anti-mitotic benzimidazole compounds. J Mol Biol168, 271-284. [PubMed]
  • Yanagida, M., Hiraoka, Y. and Katsura, I. (1983) Dynamic behaviors of DNA molecules in solution studied by fluorescence microscopy. Cold Spring Harb Symp Quant Biol47 Pt 1, 177-187. [PubMed]


  • Matsumoto, S., Morikawa, K. and Yanagida, M. (1981) Light microscopic structure of DNA in solution studied by the 4',6-diamidino-2-phenylindole staining method. J Mol Biol152, 501-516. [PubMed]
  • Morikawa, K. and Yanagida, M. (1981) Visualization of individual DNA molecules in solution by light microscopy: DAPI staining method. J Biochem (Tokyo)89, 693-696. [PubMed]
  • Morikawa, K., Yasumoto, S. and Yanagida, M. (1981) Specificity in the interaction of 4',6-diamidino-2-phenylindole with polynucleotides. Nucleic Acids Symp Ser, 85-88. [PubMed]
  • Toda, T., Yamamoto, M. and Yanagida, M. (1981) Sequential alterations in the nuclear chromatin region during mitosis of the fission yeast Schizosaccharomyces pombe: video fluorescence microscopy of synchronously growing wild-type and cold-sensitive cdc mutants by using a DNA-binding fluorescent probe. J Cell Sci52, 271-287. [PubMed]


  • Yamaguchi, Y. and Yanagida, M. (1980) Head shell protein hoc alters the surface charge of bacteriophage T4. Composite slab gel electrophoresis of phage T4 and related particles. J Mol Biol141, 175-193. [PubMed]